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Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Plasma Membrane Isolation from Eukaryotic Cell

  • Deepak B. Thimiri Govinda RajEmail author
  • Niamat Ali Khan
  • Srisaran Venkatachalam
  • Dinh Toi Chu
Part of the Methods in Molecular Biology book series


Here, we elaborate our detailed protocol for synthesis, functionalization, and application of superparamagnetic nanoparticle (SPMNP) for plasma membrane and lysosome isolation. We used standard thermal decomposition-based synthesis of iron oxide (Fe3O4) core SPMNP 1.0. Using ligand addition methodology, we surface functionalized SPMNP 1.0 with phospholipids and generated phospholipid-SPMNP 2.0. Further we used NH2-phospholipid-SPMNP 2.0 to isolate plasma membrane. Using our SPMNP subcellular fractionation protocol, we are able to isolate high-pure-high-yield plasma membrane using NH2-phospholipid-SPMNP 2.0. As a future perspective, we propose to use SPMNP on clinical patient samples and perform mass spectrometry-based proteomics, lipidomics, and glycomics for early cancer diagnosis.


Superparamagnetic nanoparticles Phospholipids Plasma membrane and pulse-chase 



This work was supported by Envirotransgene® Global. Authors thank the infrastructural support from Bannari Amman Institute of Technology, India.


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Deepak B. Thimiri Govinda Raj
    • 1
    Email author
  • Niamat Ali Khan
    • 2
  • Srisaran Venkatachalam
    • 3
  • Dinh Toi Chu
    • 4
    • 5
    • 6
  1. 1.Envirotransgene® Bio-solutions Global Chennai India and Institute of Cancer ResearchOslo University HospitalOsloNorway
  2. 2.Laboratory of Lipid Metabolism and CancerLouvainBelgium
  3. 3.Bannari Amman Institute of TechnologySathyamangalamIndia
  4. 4.Faculty of BiologyHanoi National University of EducationHanoiVietnam
  5. 5.Institute for Research and DevelopmentDuy Tan UniversityDanangVietnam
  6. 6.School of Odonto StomatologyHanoi Medical UniversityHanoiVietnam

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