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Column-Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants

  • Sherin Saheera
  • Renuka R. Nair
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2045)

Abstract

Ever since the discovery of stem cells, their isolation from tissues and expansion in culture has been extensively studied due to its potential for therapeutic application. The magnetic-assisted cell sorting (MACS) method is the most widely used technique for the sorting of cells based on their cell surface markers. Though effective, the major drawbacks are high cost and the requirement for the frequent replacement of the columns. In the column-free method, the cells are sorted using the same principle of immune-magnetic isolation but does not require magnetic columns, making it cost-effective. The isolation of c-kit+ stem cells from atrial explants using column-free magnet is found to be efficient and yields homogenous population of stem cells. This method saves time and labor and is economical when working with large sample sizes.

Keywords

Cardiac stem cells C-Kit+ Immuno-magnetic separation Column-free method 

References

  1. 1.
    He J-Q, Vu DM, Hunt G et al (2011) Human cardiac stem cells isolated from atrial appendages stably express c-kit. PLoS One 6:e27719CrossRefGoogle Scholar
  2. 2.
    Saheera S, Nair RR (2017) Accelerated decline in cardiac stem cell efficiency in spontaneously hypertensive rat compared to normotensive Wistar rat. PLoS One 12:e0189129CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Sherin Saheera
    • 1
    • 2
  • Renuka R. Nair
    • 1
  1. 1.Sree Chitra Tirunal Institute for Medical Sciences and TechnologyThiruvananthapuramIndia
  2. 2.Department of Biomedical EngineeringThe University of Alabama, BirminghamBirminghamUSA

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