pp 1-14 | Cite as
Complete Assessment of Multilineage Differentiation Potential of Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cells
The minimal criteria for mesenchymal stem/stromal cell (MSC) identification set by the International Society for Cellular Therapy include plastic adherence, presence and absence of a set of surface antigens and in vitro multilineage differentiation. This differentiation is assessed through stimulation of MSCs with defined combination and concentration of growth factors towards specific lineages and histological confirmation of the presence of differentiated cells. Here we provide protocols for multilineage differentiation, namely, osteogenesis, adipogenesis, chondrogenesis and myogenesis. We also provide their respective histological analyses.
KeywordsDifferentiation Histology Mesenchymal stem/stromal cells (MSCs) Myogenesis Skeletal muscle
This work was supported by the Slovenian Research Agency, J3-7245 Research Project and P3-0298 Research Programme.
- 1.Kalinina NI, Sysoeva VY, Rubina KA et al (2011) Mesenchymal stem cells in tissue growth and repair. Acta Nat 3:30–37Google Scholar
- 3.Friedenstein AJ, Gorskaja JF, Kulagina NN (1976) Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 4:267–274Google Scholar
- 5.Wagner W, Wein F, Seckinger A et al (2005) Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood. Exp Hematol 33:1402–1416Google Scholar
- 6.Dominici M, Le BK, Mueller I et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8:315–317Google Scholar
- 9.Wakitani S, Saito T, Caplan A (1995) Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve 18:1417–1426Google Scholar
- 15.Ruedel A, Hofmeister S, Bosserhoff A-K (2013) Development of a model system to analyze chondrogenic differentiation of mesenchymal stem cells. Int J Clin Exp Pathol 6:3042–3048Google Scholar