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pp 1-14 | Cite as

Complete Assessment of Multilineage Differentiation Potential of Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cells

  • Klemen Čamernik
  • Janja ZupanEmail author
Protocol
Part of the Methods in Molecular Biology book series

Abstract

The minimal criteria for mesenchymal stem/stromal cell (MSC) identification set by the International Society for Cellular Therapy include plastic adherence, presence and absence of a set of surface antigens and in vitro multilineage differentiation. This differentiation is assessed through stimulation of MSCs with defined combination and concentration of growth factors towards specific lineages and histological confirmation of the presence of differentiated cells. Here we provide protocols for multilineage differentiation, namely, osteogenesis, adipogenesis, chondrogenesis and myogenesis. We also provide their respective histological analyses.

Keywords

Differentiation Histology Mesenchymal stem/stromal cells (MSCs) Myogenesis Skeletal muscle 

Notes

Acknowledgements

This work was supported by the Slovenian Research Agency, J3-7245 Research Project and P3-0298 Research Programme.

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.Faculty of Pharmacy, Department of Clinical BiochemistryUniversity of LjubljanaLjubljanaSlovenia

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