pp 1-10 | Cite as
Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis
Synovium-derived mesenchymal stem/stromal cells (MSCs) have been shown to have superior features in comparison with MSCs from other tissue sources. As they are far less recognised compared to bone marrow- or adipose tissue-derived MSCs, I provide here a detailed procedure on how to isolate MSCs from human synovium. This includes determination of the proportions of viable cells in ex vivo isolated fractions before the seeding of the cells and a description of how to carry out colony-forming fibroblast assays to quantify the clonogenicity of these cells.
KeywordsColony-forming unit fibroblast assay Human Isolation Mesenchymal stem/stromal cells Synovium
Janja Zupan was funded by UK Arthritis Research in 2016–2018 and is currently part of the P3-0298 Research Programme ‘Genes, hormones and personality changes in metabolic disorders’, funded by the Slovenian Research Agency.
- 1.Sakaguchi Y, Sekiya I, Yagishita K et al (2005) Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source. Arthritis Rheum 52:2521–2529Google Scholar
- 2.De BC, Dell’Accio F, Tylzanowski P et al (2001) Multipotent mesenchymal stem cells from adult human synovial membrane. Arthritis Rheum 44:1928–1942Google Scholar
- 6.Murata Y, Uchida S, Utsunomiya H et al (2018) Synovial mesenchymal stem cells derived from the cotyloid fossa synovium have higher self-renewal and differentiation potential than those from the paralabral synovium in the hip joint. Am J Sports Med 14:1–2. https://doi.org/10.1177/0363546518794664CrossRefGoogle Scholar
- 7.Dominici M, Le BK, Mueller I et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8:315–317Google Scholar
- 8.Sacchetti B, Funari A, Remoli C et al (2016) No identical ‘mesenchymal stem cells’ at different times and sites: human committed progenitors of distinct origin and differentiation potential are incorporated as adventitial cells in microvessels. Stem Cell Reports 6:897–913. https://doi.org/10.1016/j.stemcr.2016.05.011CrossRefGoogle Scholar
- 13.Tsai C-C, Yew T-L, Yang D-C et al (2012) Benefits of hypoxic culture on bone marrow multipotent stromal cells. Am J Blood Res 2:148–159Google Scholar