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pp 1-10 | Cite as

Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis

  • Janja ZupanEmail author
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Synovium-derived mesenchymal stem/stromal cells (MSCs) have been shown to have superior features in comparison with MSCs from other tissue sources. As they are far less recognised compared to bone marrow- or adipose tissue-derived MSCs, I provide here a detailed procedure on how to isolate MSCs from human synovium. This includes determination of the proportions of viable cells in ex vivo isolated fractions before the seeding of the cells and a description of how to carry out colony-forming fibroblast assays to quantify the clonogenicity of these cells.

Keywords

Colony-forming unit fibroblast assay Human Isolation Mesenchymal stem/stromal cells Synovium 

Notes

Acknowledgements

Janja Zupan was funded by UK Arthritis Research in 2016–2018 and is currently part of the P3-0298 Research Programme ‘Genes, hormones and personality changes in metabolic disorders’, funded by the Slovenian Research Agency.

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.Faculty of Pharmacy, Department of Clinical BiochemistryUniversity of LjubljanaLjubljanaSlovenia

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