pp 1-9 | Cite as

Surface Antigen-Based Identification of In Vitro Expanded Skeletal Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry

  • Klemen Čamernik
  • Janja ZupanEmail author
Part of the Methods in Molecular Biology book series


Mesenchymal stem/stromal cells (MSCs) can be isolated from several connective tissues in the adult organism by harnessing their propensity for plastic adherence in vitro. Upon culture expansion, the resulting cell cultures are composed of many different cell types at different stages of differentiation. Hence, their identity must be confirmed. Flow cytometry is an indispensable method for accurate quantification of MSC surface antigens. Here, we present a protocol that uses flow cytometry for the identification of MSCs based on the set of surface antigens required by the International Society for Cellular Therapy.


Flow cytometry Identification Mesenchymal stem/stromal cells Skeletal muscle Surface markers 



This work was supported by the Slovenian Research Agency, J3-7245 Research Project and P3-0298 Research Programme.


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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.Faculty of Pharmacy, Department of Clinical BiochemistryUniversity of LjubljanaLjubljanaSlovenia

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