Human-Scale Liver Harvest and Decellularization for Preclinical Research
Decellularized scaffolds are promising for organ regeneration; to facilitate analyses of decellularization and preclinical research, experiments using large animals are indispensable. An efficient protocol using large animals requires the modification of current methods to account for differences in anatomy, size, stiffness, and animal handling. This chapter describes a method for the decellularization of livers from large animals (especially porcine livers), including anesthesia, organ harvest, organ decellularization, and storage, with a summary of potential pitfalls. This methodology can hypothetically be applied to large animals for preclinical studies and subsequent clinical applications.
KeywordsAcellular scaffold Bioengineering Decellularization Extracellular matrix Human scale Porcine liver Preclinical trial Regeneration therapy
This work was supported by grants from the Japan Agency for Medical Research and Development, Research Center Network for Realization of Regenerative Medicine, and Projects for Technological Development (to Y.K. and H.Y.).
- 8.Uygun BE, Soto-Gutierrez A, Yagi H, Izamis ML, Guzzardi MA, Shulman C, Milwid J, Kobayashi N, Tilles A, Berthiaume F, Hertl M, Nahmias Y, Yarmush ML, Uygun K (2010) Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix. Nat Med 16:814CrossRefGoogle Scholar
- 9.Yagi H, Fukumitsu K, Fukuda K, Kitago M, Shinoda M, Obara H, Itano O, Kawachi S, Tanabe M, Coudriet GM, Piganelli JD, Gilbert TW, Soto-Gutierrez A, Kitagawa Y (2013) Human-scale whole-organ bioengineering for liver transplantation: a regenerative medicine approach. Cell Transplant 22:231CrossRefGoogle Scholar
- 11.Struecker B, Hillebrandt KH, Voitl R, Butter A, Schmuck RB, Reutzel-Selke A, Geisel D, Joehrens K, Pickerodt PA, Raschzok N, Puhl G, Neuhaus P, Pratschke J, Sauer IM (2015) Porcine liver decellularization under oscillating pressure conditions: a technical refinement to improve the homogeneity of the decellularization process. Tissue Eng Part C Methods 21:303CrossRefGoogle Scholar