Advertisement

Dental Pulp Stem Cells in Customized 3D Nanofibrous Scaffolds for Regeneration of Peripheral Nervous System

  • Siddhartha Das
  • Jayesh R. BellareEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2125)

Abstract

Dental pulp stem cells (DPSCs) are adult multipotent stem cells of neuroectodermal origin; they provide an encouraging perspective in the domain of nerve tissue engineering. DPSCs could be transplanted in biodegradable electrospun neuro-supportive scaffold (optimized in various 3D geometries like coating on the surface of titanium implant, hollow/solid tubes, etc.) for enhanced in vivo recovery of peripheral nerves. Herein, we describe the fabrication of uniform bead-free nanofibrous scaffold which supports DPSCs, proliferation, and their subsequent neural differentiation and thus could be utilized for enhanced regeneration of peripheral nervous system.

Keywords

Dental pulp stem cells Electrospun nanofibers Neural tissue engineering Peripheral nerve regeneration 

References

  1. 1.
    Gronthos S, Mankani M, Brahim J, Robey PG, Shi S (2000) Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A 97:13625–13630CrossRefGoogle Scholar
  2. 2.
    Nosrat IV, Smith CA, Mullally P, Olson L, Nosrat CA (2004) Dental pulp cells provide neurotrophic support for dopaminergic neurons and differentiate into neurons in vitro; implications for tissue engineering and repair in the nervous system. Eur J Neurosci 19:2388–2398CrossRefGoogle Scholar
  3. 3.
    Huang AHC, Snyder BR, Cheng PH, Chan AW (2008) Putative dental pulp-derived stem/stromal cells promote proliferation and differentiation of endogenous neural cells in the hippocampus of mice. Stem Cells 26:2654–2663CrossRefGoogle Scholar
  4. 4.
    Sakai K, Yamamoto A, Matsubara K, Nakamura S, Naruse M, Yamagata M, Sakamoto K, Tauchi R, Wakao N, Imagama S (2012) Human dental pulp-derived stem cells promote locomotor recovery after complete transection of the rat spinal cord by multiple neuro-regenerative mechanisms. J Clin Invest 122:80–90PubMedGoogle Scholar
  5. 5.
    Nosrat IV, Widenfalk J, Olson L, Nosrat CA (2001) Dental pulp cells produce neurotrophic factors, interact with trigeminal neurons in vitro, and rescue motoneurons after spinal cord injury. Dev Biol 238:120–132CrossRefGoogle Scholar
  6. 6.
    Robinson LR (2000) Traumatic injury to peripheral nerves. Muscle Nerve 23:863–873CrossRefGoogle Scholar
  7. 7.
    Ciardelli G, Chiono V (2006) Materials for peripheral nerve regeneration. Macromol Biosci 6:13–26CrossRefGoogle Scholar
  8. 8.
    Evans GR, Brandt K, Katz S, Chauvin P, Otto L, Bogle M, Wang B, Meszlenyi RK, Lu L, Mikos AG (2002) Bioactive poly (L-lactic acid) conduits seeded with Schwann cells for peripheral nerve regeneration. Biomaterials 23:841–848CrossRefGoogle Scholar
  9. 9.
    Daly W, Yao L, Zeugolis D, Windebank A, Pandit A (2011) A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery. J R Soc Interface 9:202–221CrossRefGoogle Scholar
  10. 10.
    Bhatia SK (2010) Biomaterials for clinical applications. Springer Science & Business MediaGoogle Scholar
  11. 11.
    Chen X, Wang XD, Chen G, Lin WW, Yao J, Gu XS (2006) Study of in vivo differentiation of rat bone marrow stromal cells into schwann cell-like cells. Microsurgery 26:111–115CrossRefGoogle Scholar
  12. 12.
    Lopes FRP, de Moura CLC, Corrêa JD Jr, Balduino A, Lora S, Langone F, Borojevic R, Martinez AMB (2006) Bone marrow stromal cells and resorbable collagen guidance tubes enhance sciatic nerve regeneration in mice. Exp Neurol 198:457–468CrossRefGoogle Scholar
  13. 13.
    Wang D, Liu X-L, Zhu J-K, Jiang L, Hu J, Zhang Y, Yang L-M, Wang H-G, Yi J-H (2008) Bridging small-gap peripheral nerve defects using acellular nerve allograft implanted with autologous bone marrow stromal cells in primates. Brain Res 1188:44–53CrossRefGoogle Scholar
  14. 14.
    Hou S-Y, Zhang H-Y, Quan D-P, Liu X-L, Zhu J-K (2006) Tissue-engineered peripheral nerve grafting by differentiated bone marrow stromal cells. Neuroscience 140:101–110CrossRefGoogle Scholar
  15. 15.
    Choi B-H, Zhu S-J, Kim B-Y, Huh J-Y, Lee S-H, Jung J-H (2005) Transplantation of cultured bone marrow stromal cells to improve peripheral nerve regeneration. Int J Oral Maxillofac Surg 34:537–542CrossRefGoogle Scholar
  16. 16.
    Gilmore JL, Yi X, Quan L, Kabanov AV (2008) Novel nanomaterials for clinical neuroscience. J Neuroimmune Pharm 3:83–94CrossRefGoogle Scholar
  17. 17.
    Ghasemi-Mobarakeh L, Prabhakaran MP, Morshed M, Nasr-Esfahani M-H, Ramakrishna S (2008) Electrospun poly (ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials 29:4532–4539CrossRefGoogle Scholar
  18. 18.
    Bender MD, Bennett JM, Waddell RL, Doctor JS, Marra KG (2004) Multi-channeled biodegradable polymer/CultiSpher composite nerve guides. Biomaterials 25:1269–1278CrossRefGoogle Scholar
  19. 19.
    Waddell RL, Marra KG, Collins KL, Leung JT, Doctor JS (2003) Using PC12 cells to evaluate poly (caprolactone) and collagenous microcarriers for applications in nerve guide fabrication. Biotechnol Prog 19:1767–1774CrossRefGoogle Scholar
  20. 20.
    Colter DC, Sekiya I, Prockop DJ (2001) Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proc Natl Acad Sci U S A 98:7841–7845CrossRefGoogle Scholar
  21. 21.
    Mareschi K, Novara M, Rustichelli D, Ferrero I, Guido D, Carbone E, Medico E, Madon E, Vercelli A, Fagioli F (2006) Neural differentiation of human mesenchymal stem cells: evidence for expression of neural markers and eag K+ channel types. Exp Hematol 34:1563–1572CrossRefGoogle Scholar
  22. 22.
    Das S, Gurav S, Soni V, Ingle A, Mohanty BS, Chaudhari P, Bendale K, Dholam K, Bellare JR (2018) Osteogenic nanofibrous coated titanium implant results in enhanced osseointegration: in vivo preliminary study in a rabbit model. Tissue Eng Regen Med 15:231–247CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.Department of Biosciences and BioengineeringIndian Institute of Technology BombayMumbaiIndia
  2. 2.Department of Chemical EngineeringIndian Institute of Technology BombayMumbaiIndia
  3. 3.Wadhwani Research Center for Bioengineering (WRCB)Indian Institute of Technology BombayMumbaiIndia

Personalised recommendations