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Functional Assays of Stem Cell Properties Derived from Different Niches

  • Beatriz de Lucas
  • Laura M. Pérez
  • Beatriz G. Gálvez
Protocol
Part of the Methods in Molecular Biology book series

Abstract

It has been described that adult tissues contain mesenchymal stem cell populations. The specific areas where stem cells reside are known as niches. Crosstalk between cells and their niche is essential to maintain the correct functionality of stem cell. MSCs present a set of abilities such as migration, invasion, and angiogenic potentials, which make them ideal candidates for cell-based therapies. In order to test the regenerative capacity of these cells, we have described a methodology for the collection and for the evaluation of these mesenchymal precursors from different niches.

Keywords

Angiogenesis Explant Invasion Mesenchymal stem cell Migration Niche 

Notes

Acknowledgments

This study was supported by the grant from the Spanish Ministry of Science and Innovation (SAF 2015–67911-R) to BGG. BdL is supported by FPU fellowships from the Spanish Ministry of Science and Innovation.

BdL and LMP wrote the manuscript. BGG revised the manuscript. All authors read and approved the final manuscript. We would like to thank all past and present members of the “BGG group”, who have contributed to the design and evolution of these protocols over several years.

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Beatriz de Lucas
    • 1
    • 2
  • Laura M. Pérez
    • 1
    • 2
  • Beatriz G. Gálvez
    • 1
    • 2
  1. 1.Universidad Europea de MadridMadridSpain
  2. 2.Instituto de Investigación Hospital 12 de Octubre (I+12)MadridSpain

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