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Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering

  • Ehsan Taghiabadi
  • Bahareh Beiki
  • Nasser Aghdami
  • Amir Bajouri
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Application of cell-based skin substitutes has recently evolved as a novel treatment for hard-to-heal wounds. Here, we focus on the development of a novel skin substitute by seeding human adipose-derived stromal cells (ASCs) on acellular human amniotic membrane (HAM). This construction is probably associated with higher rates of host cell infiltration and implanted cell engraftment. ASCs are achieved by separation of stromal cells from lipoaspirates using collagenase digestion and acellular HAM was obtained by separation of outer membrane of the chorion and removing its epithelial cells.

Keywords

Adipose-derived stromal cells Amniotic membrane Skin substitutes Stem cell Tissue engineering Wound healing 

Notes

Acknowledgments

The protocols described here were developed with the support of Royan Institute grant.

References

  1. 1.
    Litwiniuk M, Grzela T (2014) Amniotic membrane: new concepts for an old dressing. Wound Repair Regen 22:451–456Google Scholar
  2. 2.
    Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Seifalian AM (2008) Properties of the amniotic membrane for potential use in tissue engineering. Eur Cell Mater 15:88–99Google Scholar
  3. 3.
    Taghiabadi E, Nasri S, Shafieyan S, Jalili Firoozinezhad S, Aghdami N (2015) Fabrication and characterization of spongy denuded amniotic membrane based scaffold for tissue engineering. Cell J 16:476–487Google Scholar
  4. 4.
    Lo V, Pope E (2009) Amniotic membrane use in dermatology. Int J Dermatol 48:935–940Google Scholar
  5. 5.
    Hao Y, Ma DH, Hwang DG, Kim WS, Zhang F (2000) Identification of antiangiogenic and antiinflammatory proteins in human amniotic membrane. Cornea 19:348–352Google Scholar
  6. 6.
    Tseng SC, Li DQ, Ma X (1999) Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix. J Cell Physiol 179:325–335Google Scholar
  7. 7.
    Stock SJ, Kelly RW, Riley SC, Calder AA (2007) Natural antimicrobial production by the amnion. Am J Obstet Gynecol 196:255.e1–255.e6Google Scholar
  8. 8.
    Kim SS, Song CK, Shon SK, Lee KY, Kim CH, Lee MJ et al (2009) Effects of human amniotic membrane grafts combined with marrow mesenchymal stem cells on healing of full-thickness skin defects in rabbits. Cell Tissue Res 336:59–66Google Scholar
  9. 9.
    Marquez-Curtis LA, Janowska-Wieczorek A, McGann LE, Elliott JA (2015) Mesenchymal stromal cells derived from various tissues: biological, clinical and cryopreservation aspects. Cryobiology 71:181–197Google Scholar
  10. 10.
    Jin HJ, Bae YK, Kim M, Kwon SJ, Jeon HB, Choi SJ et al (2013) Comparative analysis of human mesenchymal stem cells from bone marrow, adipose tissue, and umbilical cord blood as sources of cell therapy. Int J Mol Sci 14:17986–18001Google Scholar
  11. 11.
    Faramarzi H, Mehrabani D, Fard M, Akhavan M, Zare S, Bakhshalizadeh S et al (2016) The potential of menstrual blood-derived stem cells in differentiation to epidermal lineage: a preliminary report. World J Plast Surg 5:26–31Google Scholar
  12. 12.
    Ledesma-Martinez E, Mendoza-Nunez VM, Santiago-Osorio E (2016) Mesenchymal stem cells derived from dental pulp: a review. Stem Cells Int 2016:4709572Google Scholar
  13. 13.
    Arno A, Smith AH, Blit PH, Shehab MA, Gauglitz GG, Jeschke MG (2011) Stem cell therapy: a new treatment for burns? Pharmaceuticals 4:1355–1380Google Scholar
  14. 14.
    Motamed S, Taghiabadi E, Molaei H, Sodeifi N, Hassanpour SE, Shafieyan S et al (2017) Cell-based skin substitutes accelerate regeneration of extensive burn wounds in rats. Am J Surg 214:762–769Google Scholar
  15. 15.
    Chen JY, Mou XZ, Du XC, Xiang C (2015) Comparative analysis of biological characteristics of adult mesenchymal stem cells with different tissue origins. Asian Pac J Trop Med 8:739–746Google Scholar

Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Ehsan Taghiabadi
    • 1
    • 2
  • Bahareh Beiki
    • 2
  • Nasser Aghdami
    • 1
  • Amir Bajouri
    • 1
  1. 1.Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and TechnologyACECRTehranIran
  2. 2.Skin and Stem Cell Research CenterTehran University of Medical SciencesTehranIran

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