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Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells

  • Robert M. HoffmanEmail author
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1879)

Abstract

Human hair follicle-associated pluripotent (hHAP) stem cells were isolated from the upper parts of human hair follicles. hHAP stem cells were suspended in DMEM containing 10% fetal bovine serum (FBS) where they differentiated to cardiac muscle cells as well as neurons, glial cells, keratinocytes, and smooth muscle cells. The methods of this chapter are appropriate for use of human hair follicles to produce hHAP stem cells in sufficient quantities for future heart, nerve, and spinal cord regeneration in the clinic.

Keywords

Cardiac muscle cell differentiation Hair follicle stem cells 
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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.AntiCancer Inc.San DiegoUSA
  2. 2.Department of SurgeryUniversity of California, San DiegoSan DiegoUSA

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