Isolation of Cancer Stem Cells from Squamous Cell Carcinoma
Different cancer stem cell (CSC) populations can be found in many types of cancer, including squamous cell carcinoma (SSC). Diverse reports showed that CSC play a crucial role in the relapse of different types of cancer. CSC sustains tumor growth due to their capacity to self-renew and their potential to initiate secondary tumors with metastatic cancer features. Therefore, the development of methods for the isolation of CSC is a key step to explore the mechanisms underlying CSC maintenance. In this chapter, we provide a method for isolating CSC from cutaneous SSC using immunofluorescence labeling to allow the specific purification of CSC by fluorescence-activated cell sorting (FACS). This method is based on the use of CSC membrane markers, allowing as well the isolation CSC from different mouse strains.
KeywordsCancer stem cells FACS Mouse models Skin Squamous cell carcinoma
The Perez-Moreno lab is supported by grants from the Worldwide Cancer Research UK Foundation (15-1219) and the Novo Nordisk Foundation (NNF17OC0028028).
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