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pp 1-11 | Cite as

Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells

  • Petek Korkusuz
  • Sevil Köse
  • Nilgün Yersal
  • Selin Önen
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Magnetic-activated cell sorting (MACS) is the technology that is recently used as a magnetic-based cell isolation/purification technique. This technique enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity.

Keywords

Hematopoietic stem cell MACS Skin stem cell Spermatogonial stem cell 

Notes

Acknowledgments

The experiments presented in this protocol chapter were supported by grants from Technical and Research Council of Turkey (#113S819) and Hacettepe University Research Funds (#013D04101005, #THD-2017-13430).

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Petek Korkusuz
    • 1
  • Sevil Köse
    • 2
  • Nilgün Yersal
    • 1
  • Selin Önen
    • 3
  1. 1.Hacettepe University, Faculty of Medicine, Department of Histology and EmbryologyAnkaraTurkey
  2. 2.Atilim University, Faculty of Health Sciences, Department of Nutrition and DieteticsAnkaraTurkey
  3. 3.Hacettepe University, Institute of Health Sciences, Department of Stem Cell SciencesAnkaraTurkey

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