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pp 1-10 | Cite as

Melanoblasts as Multipotent Cells in Murine Skin

  • Tsutomu Motohashi
  • Takahiro Kunisada
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Melanoblasts (MBs) are melanocyte precursors that are derived from neural crest cells (NCCs). We recently demonstrated the multipotency of MBs; they differentiate not only into pigmented melanocytes but also other NCC derivatives. We herein describe methods for the isolation of MBs from mouse skin by flow cytometry. Methods to culture isolated MBs that retain their multipotency and isolation methods for MBs using gene-modified mouse are also described.

Keywords

CD45 Flow cytometer KIT Melanoblasts Multipotency SOX10 

Notes

Acknowledgements

This study was supported by a grant from the program Grants-in-Aid for Scientific Research (C) from the Japan Society for Promotion of Science.

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  1. 1.Department of Tissue and Organ Development, Regeneration and Advanced Medical ScienceGifu University Graduate School of MedicineGifuJapan

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