Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis
The interfollicular epidermis regenerates from a heterogeneous population of basal cells undergoing either self-renewal or terminal differentiation, thereby balancing cell loss in tissue turnover or in wound repair. In this chapter, we describe a reliable and simple method for isolating interfollicular epithelial stem cells from the skin of newborn mice or from tail and ear skin of adult mice using fluorescence-activated cell sorting (FACS). We also provide a detailed protocol for culturing interfollicular epidermal stem cells and to assess their proliferative potential and self-renewing ability. These techniques are useful for directly evaluating epidermal stem cell function in normal mice under different conditions or in genetically modified mouse models.
KeywordsAdult stem cells CD34 CD71 Clonogenic assay Epidermis Flow cytometry Integrins Isolation Keratinocytes Progenitor cells
This work was supported by the Telethon grant GGP16235 and the Italian Association for Cancer Research (AIRC IG2011-N.11369).
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