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Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury

  • Jea-Young Lee
  • Roger Lin
  • Hung Nguyen
  • M. Grant Liska
  • Trenton Lippert
  • Yuji Kaneko
  • Cesar V. Borlongan
Part of the Methods in Molecular Biology book series


Traumatic brain injury (TBI) displays cognitive and motor symptoms following the initial injury which can be exacerbated by secondary cell death. Aging contributes significantly to the morbidity of TBI, with higher rates of negative neurological and behaviors outcomes. In the recent study, young and aged animals were injected intravenously with human adipose-derived mesenchymal stem cells (hADSCs) (Tx), conditioned media (CM), or vehicle (unconditioned media) following TBI. The beneficial effects of hADSCs were analyzed using various molecular and behavioral techniques. More specially, DiR-labeled hADSCs were used to observe the biodistribution of the transplanted cells. In addition, a battery of behavior tests was conducted to evaluate the neuromotor function for each treatment group and various regions of the brain were analyzed utilizing Nissl, hematoxylin and eosin (H&E), and human nuclei (HuNu) staining. Finally, flow cytometry was also performed to determine the levels of various proteins in the spleen. Here, we discuss the protocols for characterizing the histopathological and behavioral effects of transplanted stem cells in an animal model of TBI, with an emphasis on the role of aging in the therapeutic outcomes.


Aged Animal model Neurodegeneration Neurogenesis Regenerative medicine 


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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Jea-Young Lee
    • 1
  • Roger Lin
    • 1
  • Hung Nguyen
    • 1
  • M. Grant Liska
    • 1
  • Trenton Lippert
    • 1
  • Yuji Kaneko
    • 1
  • Cesar V. Borlongan
    • 2
    • 3
  1. 1.Department of Neurosurgery and Brain Repair, Center of Excellence for Aging and Brain RepairUniversity of South Florida Morsani College of MedicineTampaUSA
  2. 2.Center of Excellence for Aging & Brain Repair, Morsani College of MedicineUniversity of South FloridaTampaUSA
  3. 3.Department of Neurosurgery and Brain Repair, Morsani College of MedicineUniversity of South FloridaTampaUSA

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