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In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering

  • Andrea PelosoEmail author
  • Antonio Citro
  • Valeria Corradetti
  • Szandra Brambilla
  • Graziano Oldani
  • Francesco Calabrese
  • Tommaso Dominioni
  • Marcello Maestri
  • Lorenzo Cobianchi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1577)

Abstract

Whole-organ decellularization is recently gaining interest in the transplantation field as strategy to obtain acellular scaffold only composed by extracellular matrix. These structures, that still remain organ-specific in terms of biological cues and tridimensional morphology could be then recellularized with patient's autologous cells. The final result should be a nwe transplantable autologous organ that should by-pass, at the same time, the problem of organ shortage and secondly the consequences related to the immunosuppression need. Herein we describe the protocol to manufacture a whole-organ transplantable rat kidney scaffold by a dual-detergent (Triton X-100 and SDS) arterial peristaltic perfusion. Final results show whole-renal acellular scaffold with contextual preservation of tridimensional architecture and biological properties deriving from the extracellular matrix composition.

Keywords:

Extracellular matrix Kidney scaffold Kidney transplantation Organ bioengineering Rat model Regenerative medicine 

Notes

Acknowledgments

AP was supported by an Investigator Fellowship grant from Collegio Ghislieri, Pavia, Italy.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Andrea Peloso
    • 1
    • 2
    • 3
    Email author
  • Antonio Citro
    • 4
  • Valeria Corradetti
    • 5
  • Szandra Brambilla
    • 6
  • Graziano Oldani
    • 3
  • Francesco Calabrese
    • 2
  • Tommaso Dominioni
    • 2
  • Marcello Maestri
    • 2
  • Lorenzo Cobianchi
    • 1
    • 2
  1. 1.Department of Clinical, Surgical, Diagnostic and Paediatric SciencesUniversity of PaviaPaviaItaly
  2. 2.Department of General SurgeryIRCCS Policlinico San MatteoPaviaItaly
  3. 3.Divisions of Abdominal and Transplantation Surgery, Hepato-Pancreato-Biliary Centre, Department of Surgery, Faculty of MedicineUniversity Hospital of Geneva, University of GenevaGenevaSwitzerland
  4. 4.San Raffaele Diabetes Research Institute, IRCCS San Raffaele Scientific InstituteMilanItaly
  5. 5.IRCCS Policlinico San Matteo, Unit of Nephrology, Dialysis and TransplantationPaviaItaly
  6. 6.University of PaviaPaviaItaly

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