Straightforward Protocol for Gel-Free Proteomic Analysis of Adipose Tissue

  • Yvonne Pasing
  • Armin Schniers
  • Terkel HansenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1788)


After conducting systematic and quantitative comparisons of different sample preparation techniques regarding their capability to efficiently and reproducibly recover proteins from biopsies, we present here our superior protocol for extracting proteins from low amounts of adipose tissue. Adipose tissue as a matrix in bottom-up proteomics is challenging due to the extremely high lipid content.

The lysis buffer utilized contains the detergent sodium deoxycholate, which does not impair the activity of trypsin and therefore enables direct digestion without detergent removal steps. The resulting workflow is time saving, cost efficient, easy to perform, and it can also be applied to other hydrophobic samples.


Adipose tissue proteome Detergents Hydrophobic samples Sample preparation Sodium deoxycholate Sodium laurate 



We would like to thank Prof. Rolf Jorde, Sayda Colnoe, Allan Didriksen, and Jack-Ansgar Bruun. Our work was supported by a grant from the North Norway Regional Health Authorities.


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Copyright information

© Springer Science+Business Media New York  2017

Authors and Affiliations

  1. 1.Tromsø Endocrine Research Group, Department of Clinical MedicineUiT – The Arctic University of NorwayTromsøNorway
  2. 2.Division of Internal MedicineUniversity Hospital of North NorwayTromsøNorway
  3. 3.Natural Products and Medicinal Chemistry Research Group, Department of PharmacyUiT – The Arctic University of NorwayTromsøNorway

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