Autophagy in Adipocyte Differentiation

  • Zhipeng Tao
  • Longhua Liu
  • Louise D. Zheng
  • Zhiyong ChengEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1854)


Adipose tissue regulates metabolic homeostasis by acting as an endocrine organ and energy reservoir. Adipose tissue development and functional maintenance are dependent on adipocyte differentiation, in which autophagy plays an important role. It has been shown that autophagy deficiency dampens adipocyte differentiation, compromises adipose tissue development, dysregulates adipocytokine secretion, and even causes sudden death in young animals. Therefore, accurate assessment of autophagy in adipocyte is critical for the study of adipose biology or pathology of metabolic diseases. In this chapter, we described the procedure of autophagy analysis during adipocyte differentiation, and discussed the power of steady-state autophagy protein (e.g., beclin 1, LC3, and p62) levels versus autophagy flux to reflect autophagy activity.


Adipocyte differentiation Adipose development and function Autophagy Endocrine Metabolism 



This work was supported in part by USDA National Institute of Food and Agriculture Hatch Project 1007334 (ZC).


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Zhipeng Tao
    • 1
  • Longhua Liu
    • 1
    • 2
  • Louise D. Zheng
    • 1
  • Zhiyong Cheng
    • 1
    Email author
  1. 1.Department of Human Nutrition, Foods, and ExerciseFralin Translational Obesity Research Center, College of Agriculture and Life ScienceBlacksburgUSA
  2. 2.Department of Pathology and Cell Biology, Naomi Berrie Diabetes CenterCollege of Physicians and Surgeons, Columbia UniversityNew YorkUSA

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