Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells

  • Takashi HoshibaEmail author
  • Naoki Kawazoe
  • Guoping Chen
Part of the Methods in Molecular Biology book series (MIMB, volume 1577)


The extracellular matrix (ECM) is an important extracellular microenvironmental factor that regulates stem cell differentiation. The ECM is remodeled according to stem cell differentiation progression to precisely regulate the differentiation. Thus, it is expected that the matrices mimicking native ECM surrounding differentiating cells at each differentiation stage provide a favorable microenvironment to promote stem cell differentiation. However, it is difficult to prepare matrices mimicking native ECM using chemical methods because the ECM has a complicated composition. The decellularization technique is useful to prepare such matrices. In this chapter, we described the protocol to prepare matrices mimicking native ECM surrounding cells that are differentiating from mesenchymal stem cells to either osteoblasts or adipocytes via stem cell differentiation culture and a detergent- and nuclease-based decellularization technique.


Adipogenesis Cell culture Decellularization Extracellular matrix Mesenchymal stem cells Osteogenesis 



This work was supported by a Grant-in-Aid for Young Scientists (A) (26702016) and a Grant-in-Aid for Challenging Exploratory Research (16K15209), funded by MEXT, Japan.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Takashi Hoshiba
    • 1
    • 2
    • 3
  • Naoki Kawazoe
    • 3
  • Guoping Chen
    • 3
  1. 1.Frontier Center for Organic MaterialsYamagata UniversityYamagataJapan
  2. 2.Innovative Flex Course for Frontier Organic Materials SystemsYamagata UniversityYamagataJapan
  3. 3.International Center for Materials NanoarchitectonicsNational Institute for Materials ScienceIbarakiJapan

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