Wharton’s Jelly Matrix Decellularization for Tissue Engineering Applications
Scaffolds, both natural and synthetic, used in tissue engineering provide mechanical support to cells. Tissue decellularization has been used to provide natural extracellular matrix scaffolds for tissue engineering purposes. In this chapter we focus on describing the methodology used to decellularize Wharton’s jelly matrix, the mucous connective tissue that surrounds umbilical cord vessels, to obtain decellularized Wharton’s jelly matrix (DWJM); an extracellular matrix that can be used for tissue engineering purposes. We also, briefly, describe our experience with processing DWJM for cell seeding and recellularization.
KeywordsCell seeding Decellularized Wharton’s jelly matrix Natural scaffolds Recellularization Tissue decellularization Wharton’s jelly matrix
- 1.Jadalannagari S, Converse G, McFall C, Buse E, Filla M, Villar MT, Artigues A, Mellot AJ, Wang J, Detamore MS, Hopkins RA, Aljitawi OS (2017) Decellularized Wharton’s Jelly from human umbilical cord as a novel 3D scaffolding material for tissue engineering applications. PLoS One 12(2):e0172098CrossRefGoogle Scholar
- 2.Zippel N, Schulze M, Tobiasch E (2009) Biomaterials and Mesenchymal stem cells for regenerative medicine. Recent Pat Biotechnol. doi: BIOT-ABSTRACT 08 [pii]Google Scholar
- 3.Hellstrom M, El-Akouri RR, Sihlbom C, Olsson BM, Lengqvist J, Backdahl H, Johansson BR, Olausson M, Sumitran-Holgersson S, Brannstrom M (2014) Towards the development of a bioengineered uterus: comparison of different protocols for rat uterus decellularization. Acta Biomater 10(12):5034–5042CrossRefGoogle Scholar
- 11.Ishige I, Nagamura-Inoue T, Honda MJ, Harnprasopwat R, Kido M, Sugimoto M, Nakauchi H, Tojo A (2009) Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton’s Jelly explants of human umbilical cord. Int J Hematol 90(2):261–269. doi: 10.1007/s12185-009-0377-3CrossRefPubMedGoogle Scholar