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Decellularization and Recellularization of Cartilage

  • Catherine A. Bautista
  • Bahar Bilgen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1577)

Abstract

Decellularization of cartilage enables the use of cartilage allografts or xenografts as natural scaffolds for repair and regeneration of injured cartilage. The preservation of the extracellular matrix ultrastructure of the graft makes this a promising tool for cartilage tissue engineering. We have optimized the decellularization protocol by enzymatically digesting proteoglycans while preserving the native collagen architecture. Here we describe our methods for cartilage decellularization and cell labeling for the tracking of infiltration for recellularization in detail.

Keywords

Allograft Cartilage Cell seeding Decellularization Recellularization Transplantation Viability 

Notes

Acknowledgments

We would like to acknowledge the assistance of Courtney M. Mazur in protocol development. This work was supported by Career Development Award # IK2 RX000760 from the United States (U.S.) Department of Veterans Affairs Rehabilitation Research and Development Service.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.University of CaliforniaSan DiegoUSA
  2. 2.Providence VA Medical CenterProvidenceUSA
  3. 3.Department of OrthopaedicsThe Warren Alpert Medical School of Brown University, Rhode Island HospitalProvidenceUSA

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