Decellularization and Recellularization of Cartilage
Decellularization of cartilage enables the use of cartilage allografts or xenografts as natural scaffolds for repair and regeneration of injured cartilage. The preservation of the extracellular matrix ultrastructure of the graft makes this a promising tool for cartilage tissue engineering. We have optimized the decellularization protocol by enzymatically digesting proteoglycans while preserving the native collagen architecture. Here we describe our methods for cartilage decellularization and cell labeling for the tracking of infiltration for recellularization in detail.
KeywordsAllograft Cartilage Cell seeding Decellularization Recellularization Transplantation Viability
We would like to acknowledge the assistance of Courtney M. Mazur in protocol development. This work was supported by Career Development Award # IK2 RX000760 from the United States (U.S.) Department of Veterans Affairs Rehabilitation Research and Development Service.
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