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Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry

  • Maria P. Kraemer
  • Suchismita Halder
  • Susan S. Smyth
  • Andrew J. Morris
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1697)

Abstract

Lysophosphatidic acids and sphingosine-1-phosphate are bioactive lipids that regulate diverse cellular and physiological processes through actions that are largely mediated by cell surface receptors. The roles played by these lipids in multiple disease processes make the enzymes and receptors involved in their synthesis, inactivation, and signaling attractive targets for pharmacological therapies. In this chapter we describe methods for sensitive accurate quantitation of LPA and S1P levels in biological fluids using liquid chromatography-coupled electrospray ionization tandem mass spectrometry.

Keywords

Liquid chromatography Lysophospholipids Mass spectrometry 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Maria P. Kraemer
    • 1
  • Suchismita Halder
    • 1
  • Susan S. Smyth
    • 1
  • Andrew J. Morris
    • 1
  1. 1.Gill Heart and Vascular Institute, University of Kentucky College of Medicine, Lexington Veterans Affairs Medical CenterLexingtonUSA

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