Tracheal Cartilage Isolation and Decellularization

  • Zachary Galliger
  • Angela Panoskaltsis-MortariEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1577)


Tissue decellularization allows isolation of tissue extracellular matrix components, enabling bioengineering of native tissue microenvironments with minimal antigenic components. Here we describe a method to harvest decellularized cartilage tissue from donor trachea using a series of chemical and enzymatic washes and incorporating the extracellular matrix in gelatin methacrylate hydrogels. This decellularized cartilage tissue is easily incorporated into a variety of hydrogels to create a cartilage tissue scaffold.


Cartilage Decellularization Extracellular matrix Hydrogel Trachea 



We would like to thank Daniel Sorby for his help procuring animal tissue and processing. We would also like to thank the Hormel company (Austin, MN) for their generosity, donating porcine trachea for research.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Pediatrics, Blood and Marrow Transplant ProgramUniversity of MinnesotaMinneapolisUSA

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