Decellularization of Bovine Small Intestinal Submucosa

  • Mahmut Parmaksiz
  • Ayşe Eser Elçin
  • Yaşar Murat Elçin
Part of the Methods in Molecular Biology book series (MIMB, volume 1577)


Decellularization technology promises to overcome some of the significant limitations in the regenerative medicine field by providing functional biocompatible grafts. The technique involves removal of the cells from the biological tissues or organs for further use in tissue engineering and clinical interventions. There are significant differences between decellularization protocols due to the intrinsic properties of different tissue types and purpose of use. This multistep, chemical-solution-based protocol is optimized for the preparation of decellularized bovine small intestinal submucosa (SIS).


Decellularization Bovine Small intestinal submucosa (SIS) Xenogenic biomaterials Extracellular matrix (ECM) Regenerative medicine Bioactive materials 


Competing Interests

Y.M.E. is the founder of Biovalda, Inc. (Ankara, Turkey) and has intellectual properties related to decellularized tissues.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Mahmut Parmaksiz
    • 1
  • Ayşe Eser Elçin
    • 1
  • Yaşar Murat Elçin
    • 2
    • 3
  1. 1.Tissue Engineering, Biomaterials and Nanobiotechnology LaboratoryAnkara University Faculty of Science, and Ankara University Stem Cell InstituteAnkaraTurkey
  2. 2.Biovalda Health Technologies, Inc.AnkaraTurkey
  3. 3.Tissue Engineering, Biomaterials and Nanobiotechnology LaboratoryAnkara University Faculty of ScienceAnkaraTurkey

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