Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells
Decellularized scaffolds present promising biomimetic approaches in various fields of tissue engineering. Different tissues have been selected for decellularization, among them extracellular matrix (ECM)-rich tissues such as tendons, ligaments and cartilage. The dense ECM of ligaments is particularly challenging to achieve a completely non-immunogenic ECM void of any cells. Here, the methods for decellularization adapted to ligamentous tissue of the iliotibial band (ITB) are presented along with cell isolation and several recolonization techniques using allogenic ITB-derived fibroblasts or mesenchymal stromal cells (MSCs).
Keywords:Acellularization Decellularization Explant culture Iliotibial band Ligamentocytes Mesenchymal stromal cells Recellularization
Anterior cruciate ligament
Bovine serum albumin
Dulbecco’s Minimum Essential Medium
Foetal bovine serum
Hematoxylin and eosin
Mesenchymal stromal cells
Phosphate buffered saline
Rounds per minute
Roswell Park Memorial Institute medium
Sodium dodecyl sulphate
Tris buffered saline
We would like to thank Tobias Schneider for his support. The authors acknowledge the financial support from Manfred Roth foundation. We would like to thank the University of Salzburg and the Austrian Agency for International Cooperation in Education and Research (OeAD-GmbH) for the scholarship and financial support of Mr. Clemens Gögele.
- 2.Hu R, Ren YJ, Yan L, Xiao ZH, Ding F, Li F et al (2015) A free anterolateral thigh flap and iliotibial band for reconstruction of soft tissue defects at children's feet and ankles. Injury 46:50–67Google Scholar