Preparation of Scaffolds from Decellularized Testicular Matrix

  • Yoni BaertEmail author
  • Ellen Goossens
Part of the Methods in Molecular Biology book series (MIMB, volume 1577)


Biological scaffolds composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Here we describe a simple and robust methodology for the preparation of decellularized testicular matrix (DTM) scaffolds with minimal damage to the native three-dimensional structure and tissue-specific ECM components. Such DTM scaffolds can help to gain a better insight into the molecular composition and function of testicular ECM and to develop new tissue engineering approaches to treat various types of male fertility disorders.


Testis Extracellular matrix Decellularization Scaffold In vitro spermatogenesis 



This study was supported by research grants from the Flemish League Against Cancer-Public Utility Foundation (VLK), the Scientific Research Foundation Flanders (FWO), the Vrije Universiteit Brussel, the Swedish Research Council/Finnish Academy of Science, Emil och Wera Cornells Stiftelse, the Swedish Childhood Cancer Foundation as well as through the regional agreement on medical training and clinical research (ALF) between Stockholm County Council and Karolinska Institutet. Y.B. is a postdoctoral fellow of the FWO.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Biology of the Testis, Research Laboratory for Reproduction, Genetics and Regenerative MedicineVrije Universiteit Brussel (VUB)BrusselsBelgium

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