A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration
Traditional ex vivo expansion of adult stem cells yields an insufficient quantity of less potent cells. Here we describe the fabrication of decellularized matrix deposited by synovium-derived stem cells (SDSCs). This matrix could serve as a three-dimensional expansion system to rejuvenate cells for proliferation and tissue-specific differentiation potential, which could benefit cartilage regeneration. The decellularized stem cell matrix (DSCM) might be a powerful system for tissue engineering and regeneration.
KeywordsDecellularized stem cell matrix Cell expansion Synovium-derived stem cells Chondrogenesis Cartilage tissue engineering Cartilage regeneration
We thank Suzanne Danley for editing the manuscript. This project was partially supported by Research Grants from the Musculoskeletal Transplant Foundation (MTF) and the National Institutes of Health (AR062763-01A1 and AR067747-01A1).