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Measuring Redox Status of Melanoma Cells

  • Feng Liu-Smith
  • Tatiana B. Krasieva
  • Jing Liu
  • Jiankang Liu
  • Frank L. MeyskensJr.
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Redox homeostasis plays multiple roles in essentially all aspects of cellular function, and hence, reliable methods for measuring cellular or tissue redox status are key elements in understanding the redox related signal pathways. However, in the free radical biology field, there are many controversies on the methods to measure reactive oxygen species. In this chapter we describe our experience in measuring superoxide, hydrogen peroxide, and a general redox status using redox-sensitive green fluorescence proteins (roGFPs) in human melanoma cells.

Keywords:

Reactive oxygen species Superoxide Hydrogen peroxide Redox-sensitive GFP ro1GFP ro2GFP Dihydroethidium (DHE) Dichlorofluorescin diacetate (DCFDA) Fluorometer Laser microscopy 

Notes

Acknowledgements

This study is supported by Oxnard Foundation and Waltmar Foundation to F.L.M., and Department of Medicine Allan Hubbell Education Funds (University of California Irvine) to F.L.S.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Feng Liu-Smith
    • 1
  • Tatiana B. Krasieva
    • 2
  • Jing Liu
    • 3
  • Jiankang Liu
    • 4
  • Frank L. MeyskensJr.
    • 5
  1. 1.Department of Epidemiology, Chao Family Comprehensive Cancer CenterUniversity of California Irvine School of MedicineIrvineUSA
  2. 2.Beckman Laser Institute and Medical ClinicUniversity of California IrvineIrvineUSA
  3. 3.The State Key Laboratory of Medical Genetics and School of Life SciencesCentral South UniversityChangsha, Hunan ProvinceChina
  4. 4.Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of EducationXi’an Jiaotong University School of Life Science and TechnologyXi’an, Shaangxi ProvinceChina
  5. 5.Department of Medicine, Chao Family Comprehensive Cancer CenterUniversity of California Irvine School of MedicineIrvineUSA

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