Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1341)

Abstract

Human embryonic stem cells (hESCs) are a promising source for cell replacement therapies. Parkinson’s disease is one of the candidate diseases for the cell replacement therapy since the motor manifestations of the disease are associated with the loss of dopaminergic neurons in the substantia nigra pars compacta. Stromal cell-derived inducing activity (SDIA) is the most commonly used method for the dopaminergic differentiation of hESCs. This chapter describes a simple, reliable, and scalable dopaminergic induction method of hESCs using PA6-derived adipocytes. Coculturing hESCs with PA6-derived adipocytes markedly reduces the variable outcomes among experiments. Moreover, the colony differentiation step of this method can also be used for the dopaminergic induction of mouse embryonic stem cells and NTERA2 cells as well.

Keywords:

Parkinson’s disease Human embryonic stem cells SDIA Adipocytes Dopaminergic differentiation 
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Notes

Acknowledgements

This study was supported by AXREGEN (EU Framework 7 project, 214003), the Linnaeus grant Bagadilico (Swedish Research Council, No. 349-2007-8626), FNRS-Sinergia (Swiss National Foundation, No. 125408), and Strategic Research Area MultiPark (Multidisciplinary Research in Parkinson’s Disease) at Lund University.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Experimental Medical Science, Faculty of MedicineLund UniversityLundSweden

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