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Restriction–Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus

  • Marat R. SadykovEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1373)

Abstract

Genetic manipulation is a powerful approach to study fundamental aspects of bacterial physiology, metabolism, and pathogenesis. Most Staphylococcus aureus strains are remarkably difficult to genetically manipulate as they possess strong host defense mechanisms that protect bacteria from cellular invasion by foreign DNA. In S. aureus these bacterial “immunity” mechanisms against invading genomes are mainly associated with restriction–modification systems. To date, prokaryotic restriction–modification systems are classified into four different types (Type I–IV), all of which have been found in the sequenced S. aureus genomes. This chapter describes the roles, classification, mechanisms of action of different types of restriction–modification systems and the recent advances in the biology of restriction and modification in S. aureus.

Keywords:

Staphylococcus aureus RM systems REase MTase REBASE Genetic manipulation 

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© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Pathology and Microbiology, Center for Staphylococcal ResearchUniversity of Nebraska Medical CenterOmahaUSA

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