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Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes Under Defined Conditions

  • Cathelijne W. van den Berg
  • David A. Elliott
  • Stefan R. Braam
  • Christine L. Mummery
  • Richard P. DavisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1353)

Abstract

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can differentiate to cardiomyocytes in vitro, offering unique opportunities to investigate cardiac development and disease as well as providing a platform to perform drug and toxicity tests. Initial cardiac differentiation methods were based on either inductive co-culture or aggregation as embryoid bodies, often in the presence of fetal calf serum. More recently, monolayer differentiation protocols have evolved as feasible alternatives and are often performed in completely defined culture medium and substrates. Thus, our ability to efficiently and reproducibly generate cardiomyocytes from multiple different hESC and hiPSC lines has improved significantly.

We have developed a directed differentiation monolayer protocol that can be used to generate cultures comprising ~50 % cardiomyocytes, in which both the culture of the undifferentiated human pluripotent stem cells (hPSCs) and the differentiation procedure itself are defined and serum-free. The differentiation method is also effective for hPSCs maintained in other culture systems. In this chapter, we outline the differentiation protocol and describe methods to assess cardiac differentiation efficiency as well as to identify and quantify the yield of cardiomyocytes.

Keywords

Human embryonic stem cells Human induced pluripotent stem cells Cardiac differentiation Cardiomyocyte characterization 

Notes

Acknowledgements

We thank Dorien Ward-van Oostwaard for providing cells. Financial support was from the Netherlands Institute of Regenerative Medicine (NIRM, FES0908; C.W.v.d.B., S.R.B., C.L.M.), the Netherlands Proteomics Consortium (050-040-250; R.P.D., C.L.M.), the Australian National Health and Medical Research Council (NHMRC; D.A.E.), and the European Research Council (StemCardioVasc; C.L.M.).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Cathelijne W. van den Berg
    • 1
  • David A. Elliott
    • 2
  • Stefan R. Braam
    • 3
  • Christine L. Mummery
    • 1
  • Richard P. Davis
    • 1
    Email author
  1. 1.Department of Anatomy & EmbryologyLeiden University Medical CenterLeidenThe Netherlands
  2. 2.Murdoch Childrens Research InstituteThe Royal Children’s HospitalParkvilleAustralia
  3. 3.Pluriomics BVLeidenThe Netherlands

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