Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions
From a perspective of manufacturer, procurement of bone marrow aspirates for isolation of mesenchymal stromal/stem cells (MSC) is challenging. The MSC isolated from adult donors have lower proliferation capacity than the cells isolated form young donors of pediatric age. To obtain more MSC from young healthy donors for allogeneic therapy on multiple patients, umbilical cord (UC) seems to be the best alternative. Here, we describe an easy, cost-effective and reproducible protocol of isolation of the MSC from Wharton’s Jelly (WJ) in UC.
Keywords:Umbilical cord Mesenchymal stem cells Explant culture
The study was supported by the studentship to H.B. from Saudi Arabia government.
- 1.Gottipamula S, Muttigi MS, Chaansa S, Ashwin KM, Priya N, Kolkundkar U, Sundar Raj S, Majumdar AS, Seetharam RN (2013) Large-scale expansion of pre-isolated bone marrow mesenchymal stromal cells in serum-free conditions. J Tissue Eng Regen Med. [Epub ahead of print]Google Scholar
- 2.Patrikoski M, Juntunen M, Boucher S, Campbell A, Vemuri MC, Mannerstrom B, Miettinen S (2013) Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells. Stem Cell Res Ther 4:27CrossRefPubMedCentralPubMedGoogle Scholar
- 3.Chase LG, Yang S, Zachar V, Yang Z, Lakshmipathy U, Bradford J, Boucher SE, Vemuri MC (2012) Development and characterization of a clinically compliant xeno-free culture medium in good manufacturing practice for human multipotent mesenchymal stem cells. Stem Cells Transl Med 1:750–758CrossRefPubMedCentralPubMedGoogle Scholar