Biomimetic Strategies Incorporating Enzymes into CaP Coatings Mimicking the In Vivo Environment

  • Ana M. Martins
  • Rui L. Reis
Part of the Methods in Molecular Biology book series (MIMB, volume 1202)


Biomimetic calcium phosphate coating (CaP) methodology mimics the natural biomineralization process of bone, which involves two phases: nucleation and growth. In this chapter we present a successful method to coating biodegradable natural-origin materials with biomimetic CaP layer as a strategy to incorporate enzymes with the main aim of controlling and tailoring their degradation over time. This strategy seems to enhance the bone-bonding, osteoconductive, and osteoinductive properties of natural-origin biomaterials that per se do not present these characteristics.


Natural-origin biomaterials Biomimetics CaP coatings Enzymes 



A.M.M. acknowledges “Fundação para a Ciência e Tecnologia” (FCT) for the Postdoctoral grant (SFRH/BPD/66897/2009) financed by POPH-QREN-Advanced Formation, and co-financed by Social European Fund and National Fund from MCTES.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.3B’s Research Group - Biomaterials, Biodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of MinhoGuimarãesPortugal

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