High-Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes

  • Gábor Földes
  • Maxime Mioulane
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1052)

Abstract

Human pluripotent stem cells (hPSC) are investigated as a source of authentic human cardiac cells for drug discovery and toxicological tests. Cell-based assays using automated fluorescence imaging platform and high-content analysis characterize hypertrophic and toxicity profiles of compounds in hPSC-derived cardiomyocytes (hPSC-CM) at the cellular and subcellular levels. In purified population of hPSC-CM loaded with cell tracer probe and cell death markers, both hypertrophic and toxicity profiles can be assessed in live cardiomyocyte cultures. Alternatively, in non-purified cultures of hPSC-CM, hypertrophy, proliferation, and cell death assays can be performed specifically in the cardiomyocyte subpopulation using antibodies directed against cardiac proteins and a combination of cell death- and proliferation-specific fluorescent probes.

Keywords

Human pluripotent stem cell Cardiomyocyte Hypertrophy Cell death Proliferation Automated high-content imaging 

Notes

Acknowledgements

G.F. was supported by Fondation Leducq and Hungarian Scientific Research Fund (OTKA 105555) and TÁMOP-4.2.2/B-10/1-2010-0013 Grant.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Gábor Földes
    • 1
  • Maxime Mioulane
    • 1
  1. 1.Imperial Centre for Experimental and Translational MedicineNational Heart and Lung Institute, Imperial College LondonLondonUK

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