Bioluminescence Imaging of Human Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Myocardial Infarction

  • Weijun Su
  • Liang Leng
  • Zhongchao Han
  • Zuoxiang He
  • Zongjin Li
Part of the Methods in Molecular Biology book series (MIMB, volume 1052)


Myocardial infarction is a leading cause of mortality and morbidity worldwide, and current treatments fail to address the underlying scarring and cell loss, which is a major cause of heart failure after infarction. The novel strategy, therapeutic angiogenesis and/or vasculogenesis with endothelial progenitor cells transplantation holds great promise to increase blood flow in ischemic areas, thus rebuild the injured heart and reverse the heart failure. Given the potential of self-renewal and differentiation into virtually all cell types, human embryonic stem cells (hESCs) may provide an alternate source of therapeutic cells by allowing the derivation of large numbers of endothelial cells for therapeutic angiogenesis and/or vasculogenesis of ischemic heart diseases. Moreover, to fully understand the fate of implanted hESCs or hESC derivatives, investigators need to monitor the motility of cells in living animals over time. In this chapter, we describe the application of bioluminescence reporter gene imaging to track the transplanted hESC-derived endothelial cells for treatment of myocardial infarction. The technology of inducing endothelial cells from hESCs will also be discussed.


Human embryonic stem cells Endothelial cells Myocardial infarction Bioluminescence imaging Firefly luciferase Green fluorescent protein 



This work was partially supported by grants from the National Basic Research Program of China (2011CB964903), National Natural Science Foundation of China (31071308), Tianjin Natural Science Foundation (12JCZDJC24900), NCET of State Education Ministry (NCET-12-0282) and Fundamental Research Funds for the Central Universities (65121018).


  1. 1.
    Li Z, Han Z, Wu JC (2009) Transplantation of human embryonic stem cell-derived endothelial cells for vascular diseases. J Cell Biochem 106(2):194–199. doi: 10.1002/jcb.22003 PubMedCrossRefGoogle Scholar
  2. 2.
    Wollert KC, Drexler H (2005) Clinical applications of stem cells for the heart. Circ Res 96(2):151–163. doi: 10.1161/01.RES.0000155333.69009.63 PubMedCrossRefGoogle Scholar
  3. 3.
    de Muinck ED, Thompson C, Simons M (2006) Progress and prospects: cell based regenerative therapy for cardiovascular disease. Gene Ther 13(8):659–671. doi: 10.1038/ PubMedCrossRefGoogle Scholar
  4. 4.
    Li Z, Wilson KD, Smith B, Kraft DL, Jia F, Huang M, Xie X, Robbins RC, Gambhir SS, Weissman IL, Wu JC (2009) Functional and transcriptional characterization of human embryonic stem cell-derived endothelial cells for treatment of myocardial infarction. PLoS One 4(12):e8443. doi: 10.1371/journal.pone.0008443 PubMedCrossRefGoogle Scholar
  5. 5.
    Moon SH, Kim JS, Park SJ, Lee HJ, Do JT, Chung HM (2011) A system for treating ischemic disease using human embryonic stem cell-derived endothelial cells without direct incorporation. Biomaterials 32(27):6445–6455. doi: 10.1016/j.biomaterials.2011.05.026 PubMedCrossRefGoogle Scholar
  6. 6.
    Yu J, Huang NF, Wilson KD, Velotta JB, Huang M, Li Z, Lee A, Robbins RC, Cooke JP, Wu JC (2009) nAChRs mediate human embryonic stem cell-derived endothelial cells: proliferation, apoptosis, and angiogenesis. PLoS One 4(9):e7040. doi: 10.1371/journal.pone.0007040 PubMedCrossRefGoogle Scholar
  7. 7.
    Wang L, Su W, Liu Z, Zhou M, Chen S, Chen Y, Lu D, Liu Y, Fan Y, Zheng Y, Han Z, Kong D, Wu JC, Xiang R, Li Z (2012) CD44 antibody-targeted liposomal nanoparticles for molecular imaging and therapy of hepatocellular carcinoma. Biomaterials 33(20): 5107–5114. doi: 10.1016/j.biomaterials. 2012.03.067 PubMedCrossRefGoogle Scholar
  8. 8.
    Su W, Zhou M, Zheng Y, Fan Y, Wang L, Han Z, Kong D, Zhao RC, Wu JC, Xiang R, Li Z (2011) Bioluminescence reporter gene imaging characterize human embryonic stem cell-derived teratoma formation. J Cell Biochem 112(3):840–848. doi: 10.1002/jcb.22982 PubMedCrossRefGoogle Scholar
  9. 9.
    Li Z, Suzuki Y, Huang M, Cao F, Xie X, Connolly AJ, Yang PC, Wu JC (2008) Comparison of reporter gene and iron particle labeling for tracking fate of human embryonic stem cells and differentiated endothelial cells in living subjects. Stem Cells 26(4):864–873. doi: 10.1634/stemcells.2007-0843 PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Weijun Su
    • 1
  • Liang Leng
    • 2
  • Zhongchao Han
    • 3
  • Zuoxiang He
    • 4
  • Zongjin Li
    • 5
  1. 1.Department of PathologyNankai University School of MedicineTianjinChina
  2. 2.Department of PathophysiologyNankai University School of MedicineTianjinChina
  3. 3.State Key Laboratory of HematologyChinese Academy of Medical SciencesTianjinChina
  4. 4.Department of Cardiac Nuclear ImagingPeking Union Medical College and Chinese Academy of Medical Sciences, Fuwai HospitalBeijingChina
  5. 5.Department of PathophysiologyNankai UniversityTianjinChina

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