Screening of Nanoparticle Embryotoxicity Using Embryonic Stem Cells
- 1.4k Downloads
Due to the increasing use of engineered nanoparticles in many consumer products, rapid and economic tests for evaluating possible adverse effects on human health are urgently needed. In the present chapter the use of mouse embryonic stem cells as a valuable tool to in vitro screen nanoparticle toxicity on embryonic tissues is described. This in vitro method is a modification of the embryonic stem cell test, which has been widely used to screen soluble chemical compounds for their embryotoxic potential. The test offers an alternative to animal experimentation, reducing experimental costs and ethical issues.
KeywordsNanoparticles ENP Engineered nanoparticles Nanomaterials Embryo In vitro test Mouse embryonic stem cells
Visualization of contracting EBs at the end of the differentiation experiments (AVI 15,590 kb)
- 9.Park MV, Annema W, Salvati A, Lesniak A, Elsaesser A, Barnes C, McKerr G, Howard CV, Lynch I, Dawson KA, Piersma AH, de Jong WH (2009) In vitro developmental toxicity test detects inhibition of stem cell differentiation by silica nanoparticles. Toxicol Appl Pharmacol 240:108–116PubMedCrossRefGoogle Scholar
- 10.Pietroiusti A, Massimiani M, Fenoglio I, Colonna M, Valentini F, Palleschi G, Camaioni A, Magrini A, Siracusa G, Bergamaschi A, Sgambato A, Campagnolo L (2011) Low doses of pristine and oxidized single-wall carbon nanotubes affect mammalian embryonic development. ACS Nano 5:4624–4633PubMedCrossRefGoogle Scholar