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Patterned Polymeric Surfaces to Study the Influence of Nanotopography on the Growth and Differentiation of Mesenchymal Stem Cells

  • Cristian Pablo Pennisi
  • Vladimir Zachar
  • Trine Fink
  • Leonid Gurevich
  • Peter Fojan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1058)

Abstract

The implementation of micro- and nanotechnologies to biomaterials constitutes a unique platform to improve our understanding on microenvironmental regulation of stem cell functions. In the recent years, various methods have been developed for the fabrication of micro- and nanopatterned polymeric culture substrates, and many of these novel surfaces are opening possibilities for new applications. Here, we provide procedures for creating nanoscale topographic features on films of poly(lactic acid), a biodegradable polymer frequently used for the fabrication of tissue engineering scaffolds. In addition, we provide methods to assess the growth and differentiation of mesenchymal stem cells cultured on the substrates.

Keywords

Substrate topography Biodegradable polymers Poly(lactic acid) Cell growth Cell differentiation Mesenchymal stem cells Adipose-derived stem cells 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Cristian Pablo Pennisi
    • 1
  • Vladimir Zachar
    • 1
  • Trine Fink
    • 1
  • Leonid Gurevich
    • 1
  • Peter Fojan
    • 1
  1. 1.Laboratory for Stem Cell Research, Department of Health Science and TechnologyAalborg UniversityAalborgDenmark

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