The use of highly specific synthetic oligonucleotide (ODN) probes, which have superior hybridization kinetics and lower manufacturing costs compared to traditional genomic probes, is one approach that can be used to perform chromosome enumeration on interphase nuclei by FISH. ODNs also generally hybridize more rapidly, are more consistent, and are cheaper to manufacture. If highly repetitive sequences are targeted, properly designed synthetic ODN probes may prove to be superior reagents for rapid FISH.
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References
Matera AG, Ward DC (1992) Oligonucleotide probes for the analysis of specific repetitive DNA sequences by fluorescence in situ hybridization. Hum Mol Genet 1:535–539
Matera AG, Baldini A, Ward DC (1993) An oligonucleotide probe specific to the centromeric region of human chromosome 15. Genomics 18:729–731
Nakagome Y, Nagafuchi S, Seki S, Nakahori Y, Tamura T, Yamada M, Iwaya M (1991) A repeating unit of the DYZ1 family on the human Y chromosome consists of segments with partial male-specificity. Cytogenet Cell Genet 56:74–77
Pinkel D, Landegent J, Collins C, Fuscoe J, Segraves R, Lucas J, Gray J (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc Natl Acad Sci USA 85:9138–9142
Waye JS, Willard HF (1985) Chromosome-specific alpha satellite DNA: nucleotide sequence analysis of the 2.0 kilobasepair repeat from the human X chromosome. Nucleic Acids Res 13(8):2731–2743
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Bradley, S., Zamechek, L., Aurich-Costa, J. (2009). Oligonucleotide FISH Probes. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH) — Application Guide. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70581-9_6
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