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DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples)

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Cereal Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1099))

Abstract

Deoxyribonucleic acid (DNA) extracted from endosperm can be effectively used for rapid genotyping using seed tissue, to evaluate seed quality from packaged grains and to determine the purity of milled grains. Methods outlined here are optimal procedures to isolate DNA from endosperm tissue of modern rice grains and of aged rice remains preserved between 50 and 100 years. The extracted DNA can be used to amplify regions of chloroplast genomic DNA (ctDNA), mitochondrial genomic DNA (mtDNA), and nuclear genomic DNA using standard PCR protocols. In addition, we describe an optimal procedure to process archaeological grain specimens, aged for a couple of thousand years, to isolate DNA from these ancient samples, referred to here as ancient DNA (aDNA). The aDNA can be successfully amplified by PCR using appropriate primer pairs designed specifically for aDNA amplification.

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References

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Author information

Authors and Affiliations

  1. National Institute of Aerobiological Sciences, Tsukuba, Ibaraki, Japan

    Chiaki Mutou

  2. Faculty of Humanities, Hirosaki University, Hirosaki, Aomori, Japan

    Katsunori Tanaka

  3. Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori, Japan

    Ryuji Ishikawa

Authors
  1. Chiaki Mutou
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  2. Katsunori Tanaka
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  3. Ryuji Ishikawa
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Editor information

Editors and Affiliations

  1. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Queensland, Australia

    Robert J. Henry

  2. Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Lismore, New South Wales, Australia

    Agnelo Furtado

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© 2014 Springer Science+Business Media New York

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Mutou, C., Tanaka, K., Ishikawa, R. (2014). DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples). In: Henry, R., Furtado, A. (eds) Cereal Genomics. Methods in Molecular Biology, vol 1099. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-715-0_2

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  • DOI: https://doi.org/10.1007/978-1-62703-715-0_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-714-3

  • Online ISBN: 978-1-62703-715-0

  • eBook Packages: Springer Protocols

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