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Adipose Tissue-Derived Mesenchymal Stem Cells: Isolation, Expansion, and Characterization

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Book cover Cellular Cardiomyoplasty

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1036))

Abstract

Over the last decade, cell therapy has emerged as a potentially new approach for the treatment of cardiovascular diseases. Among the wide range of cell types and sources, adipose-derived mesenchymal stem cells have shown promise, mainly due to its plasticity and remarkable paracrine-secretion capacity, largely demonstrated at the in vitro and in vivo levels. Furthermore, its accessibility and abundance, the low morbidity of the surgical procedure, its easy isolation, culture, and long-term passaging capacity added to its immunomodulatory properties that could allow its allogeneic transplantation, making it one of the most attractive candidates for clinical application. In this chapter, we will focus on the methodology for the isolation, expansion, phenotypical characterization, differentiation, and storage of the adipose-derived stem cells.

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Acknowledgement

This work was supported in part by funds from the ISCIII (RD06/0014, PI10/01621, CP09/00333), MINECO (PLE2009-0116), FP7 Program (INELPY), and the “UTE project CIMA.”

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Authors and Affiliations

  1. Laboratory of Cell Therapy, Division of Cancer, Foundation for Applied Medical Research, Clínica Universidad de Navarra, University of Navarra, Pamplona, Spain

    Miriam Araña, Pablo Aranda & Beatriz Pelacho

  2. Hematology and Area of Cell Therapy, Clínica Universidad de Navarra, University of Navarra, Pamplona, Spain

    Manuel Mazo

  3. Laboratory of Cell Therapy, Division of Cancer, Foundation for Applied Medical Research, Hematology and Area of Cell Therapy, Clínica Universidad de Navarra, University of Navarra, Pamplona, Spain

    Felipe Prosper

Authors
  1. Miriam Araña
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  2. Manuel Mazo
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  3. Pablo Aranda
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  4. Beatriz Pelacho
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  5. Felipe Prosper
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Editor information

Editors and Affiliations

  1. , Department of Surgery, East Tennessee State University, Johnson City, 37614, Tennessee, USA

    Race L. Kao

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Araña, M., Mazo, M., Aranda, P., Pelacho, B., Prosper, F. (2013). Adipose Tissue-Derived Mesenchymal Stem Cells: Isolation, Expansion, and Characterization. In: Kao, R. (eds) Cellular Cardiomyoplasty. Methods in Molecular Biology, vol 1036. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-511-8_4

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  • DOI: https://doi.org/10.1007/978-1-62703-511-8_4

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-510-1

  • Online ISBN: 978-1-62703-511-8

  • eBook Packages: Springer Protocols

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