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Two-Photon Imaging of Population Activity with Genetically Encoded Calcium Indicators in Living Flies

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Book cover Genetically Encoded Functional Indicators

Part of the book series: Neuromethods ((NM,volume 72))

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Abstract

Genetically encoded calcium indicators make it possible to track neural activity on a population-wide level. Here we describe a preparation that enables two-photon imaging of neural activity in an essentially intact fly. We present strategies to minimize motion of the brain, both in preparation technique and in apparatus design. We discuss key variables for reducing the problems of photobleaching and phototoxicity in order to collect high quality imaging data. Finally, we discuss approaches to analyze the large quantities of data that can now be readily acquired using the latest generation of genetically encoded calcium indicators.

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Acknowledgments

K.S.H. is supported by the Crick-Clay fellowship from the Watson School of Biological Sciences and predoctoral training grant 5T32GM065094 from the National Institute of General Medical Sciences. E.G. is supported by the Elisabeth Sloan Livingston fellowship from the Watson School of Biological Sciences. This work was funded by NIH grant R01 DC010403-01A1.

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Authors and Affiliations

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA

    Robert A. A. Campbell

  2. Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY, USA

    Kyle S. Honegger, Eyal Gruntman & Glenn C. Turner

Authors
  1. Robert A. A. Campbell
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  2. Kyle S. Honegger
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  3. Eyal Gruntman
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  4. Glenn C. Turner
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Corresponding author

Correspondence to Glenn C. Turner .

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Editors and Affiliations

  1. ICFC, Neurobiologie & Developpement, CNRS, UPR-3294, Avenue de la Terrasse 1, Gif-sur-Yvette, 91198, France

    Jean-René Martin

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Campbell, R.A.A., Honegger, K.S., Gruntman, E., Turner, G.C. (2012). Two-Photon Imaging of Population Activity with Genetically Encoded Calcium Indicators in Living Flies. In: Martin, JR. (eds) Genetically Encoded Functional Indicators. Neuromethods, vol 72. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-014-4_7

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  • DOI: https://doi.org/10.1007/978-1-62703-014-4_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-013-7

  • Online ISBN: 978-1-62703-014-4

  • eBook Packages: Springer Protocols

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