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Media Composition: Energy Sources and Metabolism

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Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 912))

Abstract

The preparation of defined culture media for embryo development has progressed from simple chemically defined media based on Krebs-Ringer bicarbonate, supplemented with glucose, bovine plasma albumin, antibiotics and utilizing a CO2-bicarbonate buffering system to more complete systems based around studies on the physiology and metabolism of the mammalian embryo. Although the concentration of substrates used in media can vary, there are many components that are quintessentially important for embryo development such as energy sources, that play a vital role in regulation of metabolism and hence viability. Here we describe the role of energy substrates within culture media and outline assays which can be utilized to measure embryo metabolism as a mechanism for determining embryo physiology and viability.

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, School of Pediatrics and Reproductive Health, University of Adelaide, Adelaide, SA, Australia

    Deirdre Zander-Fox & Michelle Lane

Authors
  1. Deirdre Zander-Fox
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  2. Michelle Lane
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Corresponding author

Correspondence to Michelle Lane .

Editor information

Editors and Affiliations

  1. , OB/GYN, University of Michigan, E. Catherine Street 1137, Ann Arbor, 48109-5622, Michigan, USA

    Gary D. Smith

  2. , Center for Reproductive Medicine, University of Michigan, Market Street 475, Ann Arbor, 48108, Michigan, USA

    Jason E. Swain

  3. Fertility Center of San Antonio, Medical Drive 4499, San Antonio, 78229, Texas, USA

    Thomas B. Pool

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Zander-Fox, D., Lane, M. (2012). Media Composition: Energy Sources and Metabolism. In: Smith, G., Swain, J., Pool, T. (eds) Embryo Culture. Methods in Molecular Biology, vol 912. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-971-6_6

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  • DOI: https://doi.org/10.1007/978-1-61779-971-6_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-970-9

  • Online ISBN: 978-1-61779-971-6

  • eBook Packages: Springer Protocols

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