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Visualization Techniques pp 225–268Cite as

Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

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Part of the book series: Neuromethods ((NM,volume 70))

Abstract

Methods to define circuit organization in the brain are largely based upon the axonal transport capabilities of neurons. Numerous tracers have been developed since the 1970s that are sequestered by neurons and transported through axons in either the anterograde or retrograde directions. These tracers have been widely applied and are integral to the literature that has defined the functional organization of the nervous system. Nevertheless, with few exceptions these tracers do not cross synapses and therefore cannot provide insight into the polysynaptic organization of neurons devoted to a particular function. Viral transneuronal tracers introduced in the 1980s have been developed to fill this void. These tracers exploit the abilities of neurotropic viruses to invade neurons and generate infectious progeny that cross synapses to infect other neurons within a circuit. The method has become increasingly popular with the development of recombinant strains of virus that are reduced in virulence and express unique reporters. In this chapter we provide an overview of guidelines for the use of pseudorabies virus for transneuronal tracing. This swine alpha herpesvirus is certainly the most widely used virus for circuit analysis and it has benefited extensively from technological developments that have increased its power as a tracer.

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References

  1. Peters A, Palay SL, Webster HD (1991) The fine structure of the nervous system, 3rd edn. Oxford University Press, New York

    Google Scholar 

  2. Chan-Palay V (1981) Light and electron microscopic autoradiographic techniques: radioactive amino acids, neurotransmitters, receptors, and combined methods with immunocytochemistry. In: Johnson JE (ed) Current trends in morphological techniques. CRC Press, Boca Raton, pp 53–89

    Google Scholar 

  3. Mesulam M-M (1981) Enzyme histochemistry of horseradish peroxidase for tracing neural connections with the light microscope. In: Johnson JE (ed) Current trends in morphological techniques. CRC Press, Boca Raton, pp 1–54

    Google Scholar 

  4. Schmued LC, Fallon JH (1986) Fluoro-gold: a new fluorescent retrograde axonal tracer with numerous unique properties. Brain Res 377:147–154

    Article  PubMed  CAS  Google Scholar 

  5. Gerfen CR, Sawchenko PE (1984) An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: immunohistochemical localization of axonally transported plant lectin, Phaseolus vulgaris leucoagglutinin (PHAL). Brain Res 290:219–238

    Article  PubMed  CAS  Google Scholar 

  6. Llewellyn-Smith IJ (1992) Retrograde tracers for light and electron microscopy. In: Bolam JP (ed) Experimental neuroanatomy: a practical approach. IRL Press, Oxford, pp 31–59

    Google Scholar 

  7. Smith Y (1992) Anterograde tracing with PHA-L and biocytin at the electron microscopic level. In: Bolam JP (ed) Experimental neuroanatomy: a practical approach. IRL Press, Oxford, pp 61–79

    Google Scholar 

  8. Horn AK, Buttner-Ennever JA (1990) The time course of retrograde transsynaptic transport of tetanus toxin fragment C in the oculomotor system of the rabbit after injection into extraocular eye muscles. Exp Brain Res 81:353–362

    Article  PubMed  CAS  Google Scholar 

  9. Evinger C, Erichsen JT (1986) Transsynaptic retrograde transport of fragment C of tetanus toxin demonstrated by immunohistochemical localization. Brain Res 380:383–388

    Article  PubMed  CAS  Google Scholar 

  10. Enquist LW, Husak PJ, Banfield BW, Smith GA (1998) Infection and spread of alpha herpesviruses in the nervous system. Adv Virus Res 51:237–347

    Article  PubMed  CAS  Google Scholar 

  11. Card JP (2001) Pseudorabies virus neuroinvasiveness: a window into the functional organization of the brain. Adv Virus Res 56:39–71

    Article  PubMed  CAS  Google Scholar 

  12. Enquist LW, Card JP (2003) Recent advances in the use of neurotropic viruses for circuit analysis. Curr Opin Neurobiol 13:603–606

    Article  PubMed  CAS  Google Scholar 

  13. Boldogkoi Z, Sik A, Denes A, Reichart A, Toldi J, Gerendai I, Kovacs KJ, Palkovits M (2004) Novel tracing paradigms—genetically engineered herpesviruses as tools for mapping functional circuits within the CNS: present status and future prospects. Prog Neurobiol 72:417–445

    Article  PubMed  CAS  Google Scholar 

  14. Kelly RM, Strick PL (2000) Rabies as a transneuronal tracer of circuits in the central nervous system. J Neurosci Methods 103:63–71

    Article  PubMed  CAS  Google Scholar 

  15. Kluge JP, Mare CJ (1974) Swine pseudorabies: abortion, clinical disease, and lesions in pregnant gilts infected with pseudorabies virus (Aujeszky’s disease). Am J Vet Res 35:991–995

    PubMed  CAS  Google Scholar 

  16. Fraser G, Ramachandran SP (1969) Studies of the virus of Aujeszky’s disease. I. Pathogenicity for rats and mice. J Comp Pathol 79:435–444

    Article  PubMed  CAS  Google Scholar 

  17. Hagemoser WA, Kluge JP, Hill HT (1980) Studies on the pathogenesis of pseudorabies in domestic cats following oral inoculation. Can J Comp Med 44:192–197

    PubMed  CAS  Google Scholar 

  18. Hall LB Jr, Kluge JP, Evans LE, Hill HT (1984) The effect of pseudorabies (Aujeszky’s) virus infection on young mature boars and boar fertility. Can J Comp Med 48:192–197

    PubMed  Google Scholar 

  19. McCracken RM, McFerran JB, Dow C (1973) The neural spread of pseudorabies virus in calves. J Gen Virol 20:17–28

    Article  PubMed  CAS  Google Scholar 

  20. Mettenleiter TC (2003) Pathogenesis of neurotropic herpesviruses: role of viral glycoproteins in neuroinvasion and transneuronal spread. Virus Res 92:197–206

    Article  PubMed  CAS  Google Scholar 

  21. Ramachandran SP, Fraser G (1971) Studies on the virus of Aujeszky’s disease. II. Pathogenicity for chicks. J Comp Pathol 81:55–62

    Article  PubMed  CAS  Google Scholar 

  22. Schmidt SP, Hagemoser WA, Kluge JP, Hill HT (1987) Pathogenesis of ovine pseudorabies (Aujeszky’s disease) following intratracheal inoculation. Can J Vet Res 51:326–333

    PubMed  CAS  Google Scholar 

  23. Pomeranz LE, Reynolds AE, Hengartner CJ (2005) Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine. Microbiol Mol Biol Rev 69:462–500

    Article  PubMed  CAS  Google Scholar 

  24. Mettenleiter TC, Keil GM, Fuchs W (2008) Molecular biology of animal herpesviruses. In: Mettenleiter TC, Sobrino F (eds) Animal viruses: molecular biology. Caister Academic Press, Norfolk

    Google Scholar 

  25. Mettenleiter TC (2000) Aujeszky’s disease (pseudorabies) virus: the virus and molecular pathogenesis—state of the art. Vet Res 31:99–115

    PubMed  CAS  Google Scholar 

  26. Card JP, Enquist LW (1995) Neurovirulence of pseudorabies virus. Crit Rev Neurobiol 9:137–162

    PubMed  CAS  Google Scholar 

  27. Bartha A (1961) Experimental reduction of virulence of Aujeszky’s disease virus. Magy Allatorv Lapja 16:42–45

    Google Scholar 

  28. Pickard GE, Smeraski CA, Tomlinson CC, Banfield BW, Kaufman J, Wilcox CL, Enquist LW, Sollars PJ (2002) Intravitreal injection of the attenuated pseudorabies virus PRV Bartha results in infection of the hamster suprachiasmatic nucleus only by retrograde transsynaptic transport via autonomic circuits. J Neurosci 22:2701–2710

    PubMed  CAS  Google Scholar 

  29. Smeraski CA, Sollars PJ, Ogilvie MD, Enquist LW, Pickard GE (2004) Suprachiasmatic nucleus input to autonomic circuits identified by retrograde transsynaptic transport of pseudorabies virus from the eye. J Comp Neurol 471:298–313

    Article  PubMed  Google Scholar 

  30. Klupp BG, Hengartner CJ, Mettenleiter TC, Enquist LW (2004) Complete, annotated sequence of the pseudorabies virus genome. J Virol 78:424–440

    Article  PubMed  CAS  Google Scholar 

  31. Curanovic D, Lyman MG, Bou-Abboud C, Card JP, Enquist LW (2009) Repair of the UL21 locus in pseudorabies virus Bartha enhances the kinetics of retrograde, transneuronal infection in vitro and in vivo. J Virol 83:1173–1183

    Article  PubMed  CAS  Google Scholar 

  32. Dolivo M (1980) A neurobiological approach to neurotropic viruses. Trends Neurosci 3:149–152

    Article  Google Scholar 

  33. Card JP, Enquist LW (1999) Transneuronal circuit analysis with pseudorabies viruses, vol 1. Wiley, San Diego

    Google Scholar 

  34. Sams JM, Jansen AS, Mettenleiter TC, Loewy AD (1995) Pseudorabies virus mutants as transneuronal tracers. Brain Res 687:182–190

    Article  PubMed  CAS  Google Scholar 

  35. McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083

    Article  PubMed  CAS  Google Scholar 

  36. Enquist LW (2002) Exploiting circuit-specific spread of pseudorabies virus in the central nervous system: insights into pathogenesis and circuit tracers. J Infect Dis 186:S209–S214

    Article  PubMed  Google Scholar 

  37. Song CK, Enquist LW, Bartness TJ (2005) New developments in tracing neural circuits with herpesviruses. Virus Res 111:235–249

    Article  PubMed  CAS  Google Scholar 

  38. Loewy AD (1995) Pseudorabies virus: a transneuronal tracer for neuroanatomical studies. In: Kaplitt MG, Loewy AD (eds) Viral vectors. Gene therapy and neuroscience applications. Academic, San Diego, pp 349–366

    Google Scholar 

  39. Cano G, Card JP, Rinaman L, Sved AF (2000) Connections of Barrington’s nucleus to the sympathetic nervous system. J Auton Nerv Syst 79:117–128

    Article  PubMed  CAS  Google Scholar 

  40. Cano G, Sved AF, Rinaman L, Rabin BS, Card JP (2001) Characterization of the central nervous system innervation of the rat spleen using viral transneuronal tracing. J Comp Neurol 439:1–18

    Article  PubMed  CAS  Google Scholar 

  41. Cano G, Passerin AM, Schiltz JC, Card JP, Morrison SF, Sved AF (2003) Anatomical ­substrates for the central control of sympathetic outflow to intercapsular adipose tissue during cold exposure. J Comp Neurol 460:303–326

    Article  PubMed  Google Scholar 

  42. Cano G, Card JP, Sved AF (2004) Dual viral transneuronal tracing of central autonomic circuits involved in the innervation of the two kidneys in the rat. J Comp Neurol 471:462–481

    Article  PubMed  Google Scholar 

  43. Card JP, Enquist LW, Moore RY (1999) Neuroinvasiveness of pseudorabies virus injected intracerebrally is dependent on viral concentration and terminal field density. J Comp Neurol 407:438–452

    Article  PubMed  CAS  Google Scholar 

  44. Kim J-S, Moore RY, Enquist LW, Card JP (1999) Circuit-specific co-infection of neurons in the rat central nervous system with two pseudorabies virus recombinants. J Virol 75:9521–9531

    Google Scholar 

  45. Smith GA, Gross SP, Enquist LW (2001) Herpesviruses use bidirectional fast-axonal transport to spread in sensory neurons. Proc Natl Acad Sci USA 98:3466–3470

    Article  PubMed  CAS  Google Scholar 

  46. Granstedt AE, Kuhn B, Wang S-H, Enquist LW (2010) Calcium imaging of neuronal circuits in vivo using a circuit-tracing pseudorabies virus. Cold Spring Harb Protoc. doi:10.1101/pdb.prot5410

    Google Scholar 

  47. Li Y-W, Wesselingh S, Blevins JE (1992) Projections from rabbit caudal medulla to C1 and A5 sympathetic premotor neurons, demonstrated with phaseolus leucoagglutinin and herpes simplex virus. J Comp Neurol 317:379–395

    Article  PubMed  CAS  Google Scholar 

  48. O’Donnell P, Lavin A, Enquist LW, Grace AA, Card JP (1997) Interconnected parallel circuits between rat nucleus accumbens and thalamus revealed by retrograde transynaptic transport of pseudorabies virus. J Neurosci 17:2143–2167

    PubMed  Google Scholar 

  49. Card JP, Rinaman L, Lynn RB, Lee B-H, Meade RP, Miselis RR, Enquist LW (1993) Pseudorabies virus infection of the rat central nervous system: ultrastructural characterization of viral replication, transport, and pathogenesis. J Neurosci 13:2515–2539

    PubMed  CAS  Google Scholar 

  50. LaVail JH, Carter SR, Topp KS (1993) The retrograde tracer FluoroGold interferes with the infectivity of herpes simplex virus. Brain Res 625:57–62

    Article  PubMed  CAS  Google Scholar 

  51. Aston-Jones G, Chen S, Zhu Y, Ohnishi ST (2001) A neural circuit for circadian regulation of arousal. Nat Neurosci 4:732–738

    Article  PubMed  CAS  Google Scholar 

  52. Jasmin L, Burkey AR, Card JP, Basbaum AI (1997) Transneuronal labeling of a nociceptive pathway, the spino-(trigemino-)parabrachio-amygdaloid, in the rat. J Neurosci 17:3751–3765

    PubMed  CAS  Google Scholar 

  53. Watson RE, Wiegand ST, Clough RW, Hoffman GE (1986) Use of cryoprotectant to maintain long-term peptide immunoreactivity and tissue morphology. Peptides 7:155–159

    Article  PubMed  CAS  Google Scholar 

  54. Geraghty RJ, Kummenacher C, Cohen GH, Eisenberg RJ, Spear PG (1998) Entry of alphaherpesvirues mediated by poliovirus receptor-related protein 1 and poliovirus receptor. Science 280:1618–1620

    Article  PubMed  CAS  Google Scholar 

  55. Milne RS, Connolly SA, Krummenacher C, Eisenberg RJ, Cohen GH (2001) Porcine HveC, a member of the highly conserved HveC/nectin 1 family, is a functional alphaherpesvirus receptor. Virology 281:315–328

    Article  PubMed  CAS  Google Scholar 

  56. Curanovic D, Enquist LW (2009) Directional transneuronal spread of alpha-herpesvirus infection. Future Virol 4:591–603

    Article  PubMed  Google Scholar 

  57. Card JP, Rinaman L, Schwaber JS, Miselis RR, Whealy ME, Robbins AK, Enquist LW (1990) Neurotropic properties of pseudorabies virus: uptake and transneuronal passage in the rat central nervous system. J Neurosci 10:1974–1994

    PubMed  CAS  Google Scholar 

  58. Rinaman L, Card JP, Enquist LW (1993) Spatiotemporal responses of astrocytes, ramified microglia, and brain macrophages to central neuronal infection with pseudorabies virus. J Neurosci 13:685–702

    PubMed  CAS  Google Scholar 

  59. Rinaman L, Roesch MR, Card JP (1999) Retrograde transynaptic pseudorabies virus infection of central autonomic circuits in neonatal rats. Dev Brain Res 114:207–216

    Article  CAS  Google Scholar 

  60. Rassnick S, Enquist LW, Sved AF, Card JP (1998) Pseudorabies virus induced leukocyte trafficking into rat CNS. J Virol 72:9181–9191

    PubMed  CAS  Google Scholar 

  61. Blinzinger K, Kretuzberg GW (1968) Displacement of synaptic terminals from regenerating motoneurons by microglial cells. Z Zellforsch Mikrosk Anat 85:145–157

    Article  PubMed  CAS  Google Scholar 

  62. Denes A, Boldogkoi Z, Hornyak A, Palkovits M, Kovacs KJ (2006) Attenuated pseudorabies virus-evoked rapid innate immune response in the rat brain. J Neuroimmunol 180:88–103

    Article  PubMed  CAS  Google Scholar 

  63. Boldogkoi Z, Reichart A, Toth IE, Sik A, Erdelyi F, Medveczky I, Llorens-Cortes C, Palkovits M, Lenkei Z (2002) Construction of recombinant pseudorabies viruses optimized for labeling and neurochemical characterization of neural circuitry. Mol Brain Res 109:105–118

    Article  PubMed  CAS  Google Scholar 

  64. Denes A, Boldogkoi Z, Uhereczky G, Hornyak A, Rusvai M, Palkovits M, Kovacs KJ (2005) Central autonomic control of the bone marrow: multisynaptic tract tracing by recombinant pseudorabies virus. Neuroscience 134:947–963

    Article  PubMed  CAS  Google Scholar 

  65. Card JP, Dubin JR, Whealy ME, Enquist LW (1995) Influence of infectious dose upon productive replication and transynaptic passage of pseudorabies virus in rat central nervous ­system. J Neurovirol 1:349–358

    Article  PubMed  CAS  Google Scholar 

  66. Schramm LP, Strack AM, Platt KB, Loewy AD (1993) Peripheral and central pathways regulating the kidney: a study using pseudorabies virus. Brain Res 616:251–262

    Article  PubMed  CAS  Google Scholar 

  67. Bamshad M, Song CK, Bartness TJ (1999) CNS origins of the sympathetic nervous system outflow to brown adipose tissue. Am J Physiol 45:R1569–R1578

    Google Scholar 

  68. Oldfield BJ, Giles ME, Watson A, Anderson C, Colvill LM, McKinley MJ (2002) The neurochemical characterisation of hypothalamic pathways projecting polysynaptically to brown adipose tissue in the rat. Neuroscience 110:515–526

    Article  PubMed  CAS  Google Scholar 

  69. Vahlne A, Nystrom B, Sandberg M, Hamberger A, Lycke E (1978) Attachment of herpes simplex virus to neurons and glial cells. J Gen Virol 40:359–371

    Article  PubMed  CAS  Google Scholar 

  70. Vahlne A, Svennerholm B, Sandberg M, Hamberger A, Lycke E (1980) Differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells. Infect Immun 28:675–680

    PubMed  CAS  Google Scholar 

  71. Marchand CF, Schwab ME (1987) Binding, uptake and retrograde axonal transport of herpes virus suis in sympathetic neurons. Brain Res 383:262–270

    Article  Google Scholar 

  72. Card JP, Santone DJ, Gluhovsky MY, Adelson PD (2005) Plastic reorganization of hippocampal and neocortical circuitry in experimental traumatic brain injury in the immature rat. J Neurotrauma 22:989–1002

    Article  PubMed  Google Scholar 

  73. Jansen ASP, Van Nguyen X, Karpitskiy V, Mettenleiter TC, Loewy AD (1995) Central command neurons of the sympathetic nervous system: basis of the fight-or-flight response. Science 270:253–260

    Article  Google Scholar 

  74. Banfield BW, Kaufman GD, Randall JA, Pickard GE (2003) Development of pseudorabies virus strains expressing red fluorescent proteins: new tools for multisynaptic labeling applications. J Virol 77:10106–10112

    Article  PubMed  CAS  Google Scholar 

  75. Billig I, Foris JM, Enquist LW, Card JP, Yates BJ (2000) Definition of neuronal circuitry controlling the activity of phrenic and abdominal motoneurons in the ferret using recombinant strains of pseudorabies virus. J Neurosci 20:7446–7454

    PubMed  CAS  Google Scholar 

  76. DeFalco J, Tomishima MJ, Liu H, Zhao C, Cai X, Marth JD, Enquist LW, Friedman JM (2001) Virus-assisted mapping of neural inputs to a feeding center in the hypothalamus. Science 291:2608–2613

    Article  PubMed  CAS  Google Scholar 

  77. Sauer B (1987) Functional expression of the Cre-Lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol Cell Biol 7:2087–2096

    PubMed  CAS  Google Scholar 

  78. Ekstrand MI, Enquist LW, Pomerantz RJ (2008) The alpha-herpesviruses: molecular pathfinders in nervous system circuits. Trends Mol Med 14:134–140

    Article  PubMed  CAS  Google Scholar 

  79. Yoon H, Enquist LW, Dulac C (2005) Olfactory inputs to hypothalamic neurons controlling reproduction and fertility. Cell 123:669–682

    Article  PubMed  CAS  Google Scholar 

  80. Campbell RE, Herbison AE (2007) Definition of brainstem afferents to gonadotropin-­releasing hormone neurons in the mouse using conditional viral tract tracing. Endocrinology 148:5884–5890

    Article  PubMed  CAS  Google Scholar 

  81. Campbell RE, Herbison AE (2007) Defining the gonadotrophin-releasing hormone neuronal network: transgenic approaches to understanding neurocircuitry. J Neuroendocrinol 19:561–573

    Article  PubMed  CAS  Google Scholar 

  82. Braz JM, Enquist LW, Basbaum AI (2009) Inputs to serotonergic neurons revealed by conditional viral transneuronal tracing. J Comp Neurol 514:145–160

    Article  PubMed  Google Scholar 

  83. Boldogkoi Z, Balint K, Awatramani GB, Balya D, Busskamp V, Viney TJ, Lagali PS, Duebel J, Pasti E, Tombacz D, Toth JS, Takacs IF, Scherf BG (2009) Genetically timed, activity-sensor and rainbow transsynaptic viral tools. Nat Methods 6:127–130

    Article  PubMed  CAS  Google Scholar 

  84. Granstedt AE, Szpara ML, Kuhn B, Wang S-H, Enquist LW (2009) Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus. PLoS One 4:e6923. doi:6910.1371/journal.pone.0006923

    Article  PubMed  Google Scholar 

  85. Card JP, Kobiler O, McCambridge J, Ebdlahad S, Shan Z, Raizada MK, Sved AF, Enquist LW (2011) Microdissection of neural networks by conditional reporter expression from a Brainbow herpesvirus. Proc Natl Acad Sci USA 108:3377–3382

    Article  PubMed  CAS  Google Scholar 

  86. Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, Lichtman JW (2007) Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450:56–63

    Article  PubMed  CAS  Google Scholar 

  87. Kobiler O, Lipman Y, Therkelsen K, Daubechies I, Enquist LW (2010) Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes. Nat Commun 1:146

    Article  PubMed  Google Scholar 

  88. Card JP (1998) Exploring brain circuitry with neurotropic viruses: new horizons in neuroanatomy. Anat Rec 253:176–185

    Article  PubMed  CAS  Google Scholar 

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  1. Department of Neuroscience, University of Pittsburgh, Pittsburgh, PA, USA

    J. Patrick Card

  2. Department of Molecular Biology, Princeton University, Princeton, NJ, USA

    Lynn W. Enquist

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Correspondence to J. Patrick Card .

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  1. School of Medical Sciences, Pharmaceutical Sciences, RMIT University, Plenty Road, Bundoora, 3183, Victoria, Australia

    Emilio Badoer

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Card, J.P., Enquist, L.W. (2012). Use and Visualization of Neuroanatomical Viral Transneuronal Tracers. In: Badoer, E. (eds) Visualization Techniques. Neuromethods, vol 70. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-897-9_11

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