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Evaluation of Innate Immune Signaling Pathways in Transformed Cells

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Book cover Oncolytic Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 797))

Abstract

Oncolytic viruses, the use of viruses to treat cancer, is emerging as a new option for cancer therapy. Oncolytic viruses, of both DNA and RNA origin, exhibit the ability to preferentially replicate in and kill cancer cells plausibly due to defects in innate immune signaling or translation regulation that are acquired during cellular transformation. Here, we review concepts and assays that describe how to analyze signaling pathways that govern the regulation of Type I IFN production as well as the induction of interferon-stimulated antiviral genes, events that are critical for mounting an effective antiviral response. The following procedures can be used to assess whether innate immune pathways that control antiviral host defense are defective in tumor cells – mechanisms that may help to explain viral oncolysis.

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Authors and Affiliations

  1. University of Miami School of Medicine, Miami, FL, USA

    Joshua F. Heiber & Glen N. Barber

Authors
  1. Joshua F. Heiber
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  2. Glen N. Barber
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Corresponding author

Correspondence to Glen N. Barber .

Editor information

Editors and Affiliations

  1. Jennerex Biotherapeutics, Inc., Market Street 1, San Francisco, 94105, California, USA

    David H. Kirn

  2. School of Medicine, Dept. Pathology & Immunology, Washington University, S. Euclid Ave. 660, St.Louis, 63110, Missouri, USA

    Ta-Chiang Liu

  3. Hillman Cancer Center, University of Pittsburgh, Centre Avenue 5117, Pittsburgh, 15213, Pennsylvania, USA

    Stephen H. Thorne

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Heiber, J.F., Barber, G.N. (2012). Evaluation of Innate Immune Signaling Pathways in Transformed Cells. In: Kirn, D., Liu, TC., Thorne, S. (eds) Oncolytic Viruses. Methods in Molecular Biology, vol 797. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-340-0_15

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  • DOI: https://doi.org/10.1007/978-1-61779-340-0_15

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  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-61779-339-4

  • Online ISBN: 978-1-61779-340-0

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