Abstract
A comprehensive knowledge of the molecular biology underlying osteogenic differentiation in a controlled, laboratory setting may promise optimization of future cell-based tissue engineering strategies for clinical problems. The scope of this review encompasses a discussion of the methodology utilized to perform such studies. Our laboratory routinely performs both in vitro and in vivo assays underlying osteogenic differentiation, and the widespread use of singular methodology across multiple investigators and institutions promises great advancements for the skeletal tissue engineering community.
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Cowan, C.M., Shi, Y.Y., Aalami, O.O. et al. Adipose-derived adult stromal cells heal critical-size mouse calvarial defects. Nat Biotechnol 22, 560–7 (2004).
Acknowledgments
This work was supported by NIH R01 DE014526, NIH R21 DE019274, and grants from the Oak and Hagey Foundations to M.T.L., NIH F32 AR055871, Stanford University Dean’s Award and Lucile Packard Children’s Health Research Program Award to D.M.G., and an American College of Surgeons Resident Research Award and Lucile Packard Children’s Health Research Program Award to N.J.P.
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© 2011 Springer Science+Business Media, LLC
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Gupta, D.M., Panetta, N.J., Longaker, M.T. (2011). Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells. In: Vemuri, M., Chase, L., Rao, M. (eds) Mesenchymal Stem Cell Assays and Applications. Methods in Molecular Biology, vol 698. Humana Press. https://doi.org/10.1007/978-1-60761-999-4_16
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DOI: https://doi.org/10.1007/978-1-60761-999-4_16
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