Abstract
The GC or GC-MS method discussed in Chapter 102 can distinguish substituted monosaccharides, but to characterize the position of acyl groups together with the linkages between the monosaccharides, a strategy has been developed to “capture” the substitution pattern by methyiation of all free hydroxyl groups. The constituent monosaccharides are then analyzed after hydrolysis, reduction, and acetylation as partially methylated alditol acetates in a procedure known as methyiation analysis (1–3).
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References
Hansson, G. C. and Karlsson, H. (1993) Gas chromatography and gas chromatography-mass spectrometry of glycoprotein oligosaccharides, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 47–54.
Güther, M. L. and Ferguson, M. A. J. (1993) The microanalysis of glycosyl phosphatidylinositol glycans, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 99–117.
Hounsell, E. F. (1994) Physicochemical analysis of oligosaccharide determinants of glycoproteins. Adv. Carbohyd. Chem. Biochem. 50, 311–350.
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© 1996 Humana Press Inc., Totowa, NJ
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Hounsell, E.F., Davies, M.J., Smith, K.D. (1996). Determination of Monosaccharide Linkage and Substitution Patterns by GC-MS Methyiation Analysis. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_103
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DOI: https://doi.org/10.1007/978-1-60327-259-9_103
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