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Oligonucleotide Microarrays for Identification of Microbial Pathogens and Detection of Their Virulence-Associated or Drug-Resistance Determinants

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Biological Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 671))

Abstract

Microarrays are spatially ordered arrays with ligands chemically immobilized in discrete spots on a solid matrix, usually a microscope slide. Microarrays are a high-throughput large-scale screening system enabling simultaneous identification of a large number of labeled target molecules (up to several hundred thousand) that bind specifically to the immobilized ligands of the array. DNA microarrays represent a promising tool for clinical, environmental, and industrial microbiology since the technology allows relatively rapid identification of large number of genetic determinants simultaneously, providing detailed genomic level information regarding the pathogen species, including identification of their virulence-associated factors and the presence of antibiotic resistance genes. In this chapter, we describe key aspects and methodologies important for the development and use of DNA microarrays for microbial diagnostics.

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Author information

Authors and Affiliations

  1. Center for Biologics Evaluation and Research, Food and Drug Administration, Kensington, MD, USA

    Dmitriy V. Volokhov, Hyesuk Kong & Vladimir E. Chizhikov

  2. Department of Bioengineering, University of Maryland, College Park, MD, USA

    Keith Herold

  3. National Institutes of Health, National Cancer Institute, FDA, Bethesda, MD, USA

    Avraham Rasooly

Authors
  1. Dmitriy V. Volokhov
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  2. Hyesuk Kong
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  3. Keith Herold
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  4. Vladimir E. Chizhikov
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  5. Avraham Rasooly
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Editor information

Editors and Affiliations

  1. , MIT Division of Health Sciences and Tech, Harvard Medical School, Landsdowne St. 65, Cambridge, 02139, Massachusetts, USA

    Ali Khademhosseini

  2. , Department of Mechanical and Aerospace E, Seoul National University, Kwanak-gu, Shinlim-dong 56-1-1, Seoul, 151-742, Korea, Republic of (South Korea)

    Kahp-Yang Suh

  3. Biophage Pharma, Inc., Royalmount Ave. 6100, Montreal, H4P 2R2, Québec, Canada

    Mohammed Zourob

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Volokhov, D.V., Kong, H., Herold, K., Chizhikov, V.E., Rasooly, A. (2011). Oligonucleotide Microarrays for Identification of Microbial Pathogens and Detection of Their Virulence-Associated or Drug-Resistance Determinants. In: Khademhosseini, A., Suh, KY., Zourob, M. (eds) Biological Microarrays. Methods in Molecular Biology, vol 671. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-551-0_3

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  • DOI: https://doi.org/10.1007/978-1-59745-551-0_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-95-4

  • Online ISBN: 978-1-59745-551-0

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