Abstract
During meiosis Spo11 catalyzes the formation of DNA double-strand breaks, becoming covalently attached to the 5′ ends on both sides of the break during this process. Spo11 is removed from the DSB by single-stranded endonucleolytic cleavage flanking the DSB, liberating a short-lived species consisting of Spo11 protein covalently linked to a short oligonucleotide. The method presented here details how to detect these Spo11-oligo complexes in extracts made from meiotic yeast cells.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Neale, M.J., Keeney, S. (2009). End-Labeling and Analysis of Spo11-Oligonucleotide Complexes in Saccharomyces cerevisiae . In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 557. Humana Press. https://doi.org/10.1007/978-1-59745-527-5_12
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DOI: https://doi.org/10.1007/978-1-59745-527-5_12
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