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Protein Crystallization

  • Protocol
Book cover Cancer Genomics and Proteomics

Part of the book series: Methods in Molecularbiology™ ((MIMB,volume 383))

Abstract

X-ray crystallography is a powerful method for obtaining the three-dimensional structures of biological macromolecules and macromolecular complexes. Improvements in protein production, crystallization, data collection, as well as structure solution and refinement methods have brought the field to the verge of rapid high-throughput genomic scale structure determination. The major bottle neck to this process remains protein production and crystallization. This chapter describes essential information on standard protein production and crystallization methods and ongoing efforts to perform this work using high-throughput robotics.

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Author information

Authors and Affiliations

  1. Center for Biophysical Sciences and Engineering, Department of Vision Sciences, University of Alabama at Birmingham, Birmingham, AL

    Champion Deivanayagam

  2. Department of Pathology, University of Alabama at Birmingham, Birmingham, AL

    William J. Cook

  3. Department of Microbiology, University of Alabama in Birmingham-CBSE, Birmingham, AL

    Mark R. Walter

Authors
  1. Champion Deivanayagam
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  2. William J. Cook
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  3. Mark R. Walter
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Editor information

Editors and Affiliations

  1. Department of Pathology, Columbia University Medical Center, College of Physicians and Surgeons, New York, NY

    Paul B. Fisher

  2. Department of Neurosurgery, Columbia University Medical Center, College of Physicians and Surgeons, New York, NY

    Paul B. Fisher

  3. Department of Urology, Columbia University Medical Center, College of Physicians and Surgeons, New York, NY

    Paul B. Fisher

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© 2007 Humana Press Inc., Totowa, NJ

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Deivanayagam, C., Cook, W.J., Walter, M.R. (2007). Protein Crystallization. In: Fisher, P.B. (eds) Cancer Genomics and Proteomics. Methods in Molecularbiology™, vol 383. Humana Press. https://doi.org/10.1007/978-1-59745-335-6_22

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  • DOI: https://doi.org/10.1007/978-1-59745-335-6_22

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-504-0

  • Online ISBN: 978-1-59745-335-6

  • eBook Packages: Springer Protocols

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