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Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation

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Chromatin Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 523))

Abstract

Histone chaperones are a group of histone-interacting proteins, involved in several important cellular functions. These chaperones are essential to facilitate ordered assembly of nucleosomes, both in replication dependent and independent manner. Replication independent function of histone chaperone is necessary for histone eviction during transcriptional initiation and elongation. In this chapter we have discussed a method to evaluate the role of histone chaperone NPM1 (the only known chaperone to get acetylated with functional consequence) in the transcriptional activation which is acetylation dependent.

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Notes

  1. 1.

    The first three authors have contributed equally and Tapas K. Kundu is the corresponding author.

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Acknowledgments

We thank R. G. Roeder and J. Kadonaga for some of the valuable reagents used in this work.

Author information

Authors and Affiliations

  1. Transcription and Disease Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore, India

    Shrikanth S. Gadad, Jayasha Shandilya & Tapas K. Kundu

  2. Workman Lab, Stowers Institute for Medical Research, Kansas City, MO, USA

    Venkatesh Swaminathan

Authors
  1. Shrikanth S. Gadad
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  2. Jayasha Shandilya
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  3. Venkatesh Swaminathan
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  4. Tapas K. Kundu
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Editor information

Editors and Affiliations

  1. Research Institute, H. Lee Moffitt Cancer Center &, Magnolia Drive 12902, Tampa, 33612, U.S.A.

    Srikumar P. Chellappan

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Gadad, S.S., Shandilya, J., Swaminathan, V., Kundu, T.K. (2009). Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation. In: Chellappan, S. (eds) Chromatin Protocols. Methods in Molecular Biology, vol 523. Humana Press. https://doi.org/10.1007/978-1-59745-190-1_18

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  • DOI: https://doi.org/10.1007/978-1-59745-190-1_18

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-873-7

  • Online ISBN: 978-1-59745-190-1

  • eBook Packages: Springer Protocols

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